Disulfide Bond/Free Cysteine Detection

Disulfide bond (S-S bond) is formed by the oxidation of thiol groups (-SH) on two cysteines in proteins and is an important form of post-translational modification. Proteins are linked together by various interchain and intrachain connections of cysteines, which is crucial for maintaining the correct tertiary structure of protein molecules and preserving their biological activity. The arrangement of disulfide bonds in antibody drugs reflects the structural characteristics of the drugs; therefore, confirming the form of disulfide bond connection plays a very important role in the structural confirmation process of antibody drugs.

Bioytek offers disulfide bond detection services based on high-resolution mass spectrometry, combining years of experimental experience to provide high-level disulfide bond analysis solutions for protein drugs, including methods to block protein disulfide bond exchange in vitro while maintaining their native structures, mass spectrometry analysis methods for disulfide bond cross-linked peptides, and supporting pLink-SS software analysis for disulfide bond identification, enabling the analysis of disulfide connections and free thiols. Disulfide bond connections can be confirmed by mass spectrometry to identify the number and sites of disulfide bonds in protein drugs. Free thiols are used to confirm the proportion of free thiols in protein drugs. With years of experience, Bioytek can efficiently detect conventional disulfide bonds, overlapping cross-linked disulfide bonds, or mismatched disulfide bonds.

Determination of protein disulfide bond sites

Bioytek's disulfide bond detection can meet the following needs:

(1) Identification of the number of disulfide bonds and free thiols in protein drugs
(2) Identification of disulfide bond positions in protein drugs

Chinese/English project report

In the technical report, Bioytek will provide you with a detailed bilingual technical report in both Chinese and English, including:

1. Experimental steps (Chinese and English)
2. Relevant mass spectrometry parameters (Chinese and English)
3. Mass spectrometry images
4. Raw data
5. Information on protein disulfide bond connection sites and proportion of free cysteine

One-stop service for protein disulfide bond and free cysteine detection

You only need to place an order and send samples
Bioytek provides a one-stop service: sample processing, on-machine analysis, data analysis, project report

Related services

Biopharmaceutical analysis
Identification and quantitative analysis of protein disulfide bonds
Post-translational modification proteomics analysis
Protein structure identification