N/C Terminal Sequencing

The C-terminal sequence is an important structural and functional site for proteins and peptides, and it may even play a decisive role in the biological function of proteins. The main protein C-terminal sequencing methods are carboxypeptidase method, chemical method, and tandem mass spectrometry method. Each method has its own advantages and disadvantages. Therefore, combining multiple sequencing methods can meet the requirements of various protein sequencing. For example, the Edman degradation method cannot solve the sequencing problem of N-terminal blockage and protein modification. In such cases, mass spectrometry can be used for analysis. Protagenix Biotechnology uses ESI and MALDI TOF-based methods for N/C-terminal sequencing of samples, which can determine the C-terminal of blocked and modified proteins, complementing the Edman degradation method and ensuring the smooth progress of N and C-terminal sequencing services.

Protein N/C-terminal sequencing

Sample requirements

Protein samples, state: dry powder, solution;
Purity: total amount >300 ug, purity >90%;
Salt content: volatile inorganic salt <20 mM, non-volatile inorganic salt <5 mM

Case analysis:

Chinese/English project report

In the technical report, Protagenix will provide you with a detailed bilingual technical report in Chinese and English, including:

1. Experimental steps (Chinese and English)
2. Relevant mass spectrometry parameters (Chinese and English)
3. Mass spectrometry images
4. Raw data
5. Biopharmaceutical N/C-terminal sequence information

One-stop service for biopharmaceutical N/C-terminal sequence determination

You only need to place an order and send samples
Protagenix's one-stop service completes: sample processing - machine analysis - data analysis - project report

Related services

Biopharmaceutical analysis
Protein N/C-terminal sequencing
N-terminal sequence analysis
Protein N-terminal sequence analysis based on Edman degradation