Unlike N-glycans, O-glycans are smaller, highly diverse branched carbohydrate molecules that can attach to various protein structures. This characteristic leads to analytical complexity, making it challenging to analyze O-glycosylation sites. The variability of glycosylation sites is a key indicator of cellular activity, as evidenced by the correlation between reduced attachment of glycosylation sites on serum proteins and the severity of congenital glycosylation disorders (CDGs). Therefore, accurately determining site occupancy is crucial to fully understand the impact of protein glycosylation on human health.
The general steps for O-glycosylation site analysis include proteolytic digestion of glycoproteins, hydrophilic interaction liquid chromatography for enrichment and separation of glycopeptides. Enriched O-glycopeptides can be analyzed using reverse-phase liquid chromatography-ion trap mass spectrometry.
General process for analyzing O-glycosylation site attachment
Bio-Technology Co., Ltd. has many years of experience in glycomics analysis services and can customize glycomics analysis services according to your specific needs. Welcome to inquire!
Bilingual project report in Chinese/English
In the technical report, Bio-Technology Co., Ltd. will provide you with a detailed bilingual technical report in Chinese and English, which includes:
1. Experimental procedures (Chinese/English)
2. Relevant mass spectrometry parameters (Chinese/English)
3. Mass spectrometry images
4. Raw data
5. Detailed information on identified glycosylation sites
One-stop service for N-glycosylation site analysis
You only need to place an order and send samples
Bio-Technology Co., Ltd. completes the one-stop service: sample processing, on-machine analysis, data analysis, project report
