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Infrared Spectroscopy (FT-IR)

Fourier Transform Infrared Spectroscopy (FT-IR) is a powerful technique used to obtain the infrared spectra of absorption/emission of solids, liquids, or gases. When infrared radiation passes through a sample being measured, part of the infrared radiation is absorbed by specific covalent bonds of functional groups, while the other part directly penetrates. The collected spectrum represents the molecular absorption and transmission, forming a molecular fingerprint used for chemical identification. This also makes infrared spectroscopy applicable for various types of analyses. Fourier Transform Infrared Spectrometers simultaneously collect high-resolution spectra over a wide wavelength range, which has significant advantages compared to dispersive spectrometers, which can only measure peak intensity over a relatively narrow wavelength range at a time.

Fourier Transform Infrared Spectroscopy (FT-IR) Analysis

Fourier Transform Infrared Spectrometers can be used for all applications that use dispersive instruments to enhance sensitivity and speed, and they outperform the dispersive or filter methods of infrared spectroscopy in terms of: 1. Non-destructive nature; 2. No need for external calibration; 3. Faster speed; 4. Higher sensitivity; 5. Higher light throughput; 6. Simpler operation.

Applications of Fourier Transform Infrared Spectrometer Analysis

1. Chemical identification based on spectral differences of homogeneity, homologues, geometric and optical isomers;

2. Identify functional groups in the chemical being measured based on the absorbed wavelengths;

3. Estimate purity by studying the peaks of potential contaminants;

4. Track chemical reaction processes by comparing peaks of specific functional groups;

5. Perform quantitative analysis of chemical substances by monitoring specific peaks.

BTP Biotech, based on CNAS/ISO9001 dual quality certification system, has established seven testing platforms and uses Thermo's Nicolet series instruments to establish an FT-IR analysis platform. It measures the infrared spectra of proteins and peptides in samples and performs subsequent baseline correction, Gaussian deconvolution, and second derivative fitting to ultimately determine the secondary structure information of proteins and peptides in the sample based on peak areas.Contact us for free project consultation.

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