When analyzing peptide mixtures with MALDI-TOF, it can tolerate a certain amount of buffers and salts, and almost all peptide fragments produce only singly charged ions. Therefore, MALDI-TOF MS becomes an ideal tool for mass peptide mapping analysis. When you need to verify the theoretical sequence of protein (peptide) samples using MALDI TOF, we will first enzymatically digest the samples obtained, then confirm the peptide fingerprint map with MALDI MS, validate the peak map with MS/MS, and finally compare it with the theoretical sequence you provided to obtain the peptide coverage map. BiotechPack has developed a mass peptide mapping analysis based on MALDI TOF MS using the existing mass spectrometry technology platform.
Peptide Mapping Analysis
Advantages and Disadvantages of MALDI TOF for Mass Peptide Mapping Analysis:
Advantages:It is a classic method for protein identification, with a simple algorithm and fast speed. It was widely used before the advent of tandem mass spectrometry identification.
Disadvantages:Peptides with similar mass increase the difficulty of matching, and it is impossible to identify mixed proteins. It is not suitable for species with incomplete databases for protein identification. It cannot analyze post-translational modification sites and is not easy to connect with liquid chromatography.
Bilingual Project Report
In the technical report, BiotechPack will provide you with a detailed bilingual technical report in both Chinese and English, including:
1. Experimental Steps (Chinese and English)
2. Relevant Mass Spectrometry Parameters (Chinese and English)
3. Mass Spectrometry Images
4. Raw Data
5. Peptide Mapping Information
One-stop Service for MALDI-TOF Protein Peptide Mapping Determination
You only need to place an order and send the sample
BiotechPack completes the one-stop service: sample processing, on-machine analysis, data analysis, and project report
