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Dynamic Light Scattering Analysis (DLS)

Dynamic light scattering (DLS), also known asphoton correlation spectroscopy (PCS) orquasi-elastic light scattering (QELS), is one of the most commonly used analytical methods for determining particle size and radius distribution in suspended particles or polymers in solution samples.

Within the scope of DLS, the time fluctuations are typically analyzed through the intensity or photon autocorrelation function (ACF). A monochromatic light beam (e.g., laser) is directed onto a test solution containing spherical particles moving in Brownian motion. When the light hits the moving particles, it causes a Doppler shift, altering the wavelength of the original light. This change is related to the size of the particles. By measuring the diffusion coefficient of particles in the medium using ACF, the size distribution of the spheres can be calculated, and the movement of particles in the medium can be detailed. Additionally, DLS can be used to probe the behavior of complex fluids, such as concentrated polymer solutions.

Analysis based on Dynamic Light Scattering (DLS)

In practical applications, DLS can be used to determine the size distribution of various particles, including proteins, polymers, micelles, carbohydrates, and nanoparticles. If the system is monodisperse in size, the average effective diameter of the particles can be determined, as the measurement depends not only on the core size of the particles but also on the size of the surface structure, particle concentration, and the type of ions in the medium.

Advantages of Dynamic Light Scattering (DLS) Analysis

1. Accurate, reliable, and repeatable size analysis

2. Simple sample preparation, with the ability to analyze natural samples directly without preparation

3. Simple setup and fully automated measurements

4. Capable of measuring sizes smaller than 1nm

5. Can measure molecules with molecular weight <1000Da

6. Low volume requirement

Dynamic light scattering (DLS) analysis can obtain important parameters, such as molecular weight, radius of gyration, translational diffusion constant, etc.Feel free to contact us!

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