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Single Cell Sequencing

Single-cell sequencing technology refers to the sequencing of the genome or transcriptome of a single cell to obtain genomic, transcriptomic, or other multi-omics information, thereby revealing differences in cell populations and cell developmental lineage relationships. Traditional sequencing methods generally obtain information from mixed cell samples, which often lose cell heterogeneity information. Single-cell sequencing technology, combined with optimized NGS sequencing technology, has great advantages in detecting heterogeneity between individual cells, identifying rare cells, mapping cell charts, and more. It can help researchers study cell functions and better understand cell differentiation, cell lineage relationships, and disease evolution. Single-cell sequencing also aids in discovering potential biomarkers and drug targets, as well as analyzing rare cell types.

In 2013, 'Nature Method' listed single-cell sequencing technology as the technology of the year. Early single-cell sequencing was limited in its widespread application due to high costs. With continuous technological development, many new single-cell sequencing methods have been developed, and the application of single-cell sequencing technology in various fields is becoming more widespread, driving research on molecular mechanisms at the single-cell level.
10x Genomics Chromium is currently the optimal solution for single-cell sequencing technology. 10x Genomics Chromium provides a solution that can characterize cell features and gene expression at a single-cell level on a large scale. The 10x Genomics Chromium single-cell sequencing system can capture tens of thousands of cells into independent, traceable single-cell libraries in a short time. Each library contains an identifiable barcode for downstream analysis. The system can process 8 samples simultaneously, with each run taking about 20 minutes, allowing for multiple runs in a day. Cell sample capture is not affected by sample size, and fresh, frozen, or methanol-fixed samples can all be measured. Because 10x Genomics single-cell sequencing technology can quickly and accurately process a large number of single cells, it provides solutions for application areas such as cancer, neurology, and immunology.

10x Genomics ChromiumWidely applicable:

Gene expression analysis
Immune profiling
CRISPR screening
Copy number variation testing
Chromatin accessibility sequencing (ATAC-Seq)
Cell surface protein identification (CITE-Seq/REAP-Seq)

BaiTaiPack Biotechnology uses the 10x Genomics Chromium system combined with the Illumina platform to provide a one-stop single-cell sequencing solution. You only need to provide a single-cell suspension, and BaiTaiPack Biotechnology's single-cell sequencing service will provide you with all the services needed to obtain high-quality sequencing data, including flow cytometry sorting, library preparation, sequencing, and data analysis.Welcome to consult for free!