Proteomics Analysis
The term 'Proteomics' is derived from a combination of 'protein' and 'genomics', meaning 'the complete set of proteins expressed by a genome', which includes all the proteins expressed by a cell or even an organism. The concept of 'Proteome' was first proposed by Marc Wikins in 1995 (also reported as 1994 and 1996), and in 1997, Peter James (employed at the Swiss Federal Institute of Technology, known as the European MIT) further developed the concept of proteomics based on this. The concepts of genomics and proteomics have spurred the development of various 'omics' fields, and their emergence and development have made systems biology possible.
The study of proteomes not only provides a material basis for understanding the laws of life activities but also provides theoretical evidence and solutions for elucidating and overcoming the mechanisms of various diseases. By comparing the proteomes of normal and pathological individuals, we can identify certain 'disease-specific protein molecules', which can become molecular targets for new drug design or provide molecular markers for early disease diagnosis. Indeed, the best-selling drugs worldwide are themselves proteins or have targets that are specific protein molecules. Thus, proteomics research is not only essential for exploring the mysteries of life but also offers significant benefits for human health. Proteomics research characterizes the post-genomic era of life sciences.
Nasrin, D. et al. Iran. J. Pharm. Sci. 2018.
Applications of Proteomics
Proteomics analysis refers to the systematic identification and quantification of the entire protein set (proteome) of a biological system (cell, tissue, organ, biological fluid, or organism) at a specific time point. Mass spectrometry is the most commonly used technique for proteomics analysis. Biotech company BTP uses high-throughput mass spectrometry to establish a series of proteomics analysis platforms certified by both CNAS/ISO9001 quality systems, offering first-class mass spectrometry-based proteomics technologies and analytical capabilities, providing you with a complete set of proteomics solutions.
Protein identification is a fundamental step in proteomics aimed at recognizing the proteins present in a sample, addressing the question of whether a protein product is as expected. The molecular weight, isoelectric point, structure, and amino acid sequence of proteins are key attributes for determining protein types. BTP offers high-resolution mass spectrometry, capillary electrophoresis, circular dichroism, and other protein identification platforms, enabling precise identification from different angles to meet various research needs. Whether your sample is a protein mixture, SDS-PAGE protein band, 2D protein gel spot, or proteins in pull-down and Co-IP samples, we can provide efficient and accurate identification.Molecular Weight,Isoelectric Point,StructureProperties for precise identification, analyzing sample proteins from different perspectives to meet various research needs. Whether your sample is a protein mixture, SDS-PAGE protein band, 2D protein gel spot, or proteins from pull-down and Co-IP samples, we can achieve high-efficiency and precise identification for you.
2.Quantitative Proteome Analysis
Protein quantification is the process of determining the relative or absolute amounts of proteins in different samples. In proteomics research, researchers often need to compare protein expression levels under different conditions, such as normal versus disease states or changes at different time points. Quantification can be achieved using various methods, such as isotope labeling (TMT, iTRAQ, SILAC, 15^N labeling) and label-free quantification, to provide information on protein quantities in different biological contexts. BTP's quantitative proteomics platform offers a variety of protein quantification techniques, includingLabel Free,iTRAQ,TMT,SILAC,2D-DIGE,DIA,SWATH,Protein Relative Quantification,Semi-QuantitativeandAbsolute Quantification, to satisfy various research needs. Feel free to consult us for more information!
Protein sequencing refers to the analysis of the amino acid residue sequence that makes up the primary structure of proteins. It is fundamental to understanding the structure and biological function of proteins within organisms. BTP uses nano LC-MS/MS combined with tandem mass spectrometry and Shimadzu's Edman degradation sequencing system for protein sequence analysis, providingMass Spectrometry-Based Protein Sequencing Analysisservices, includingAmino Acid Composition Analysis,N-terminal Sequencing,C-terminal SequencingandFull Sequence Analysis, as well asEdman Degradation-Based Protein N-Terminal Sequence Analysisservices. For proteins with unknown theoretical sequences, we provideDe Novo Protein Sequencingservices for protein sequence analysis.
BiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services ProviderPost-Translational ModificationProteome Analysis
Protein post-translational modification (PTM) refers to the chemical modification process that proteins undergo after translation, affecting their function, stability, localization, and other characteristics. Common PTMs include phosphorylation, glycosylation, ubiquitination, methylation, acetylation, etc. Studying PTMs requires high-resolution mass spectrometry and other techniques to identify and quantify different modified forms of proteins in samples. BTP has advanced analytical platforms with various enrichment methods, capable of identifying low-abundance modification peptides, to characterize various PTMs, providingPhosphorylation</Tyrosine Phosphorylation</Glycosylation</Ubiquitination</Acetylation</Methylation</Disulfide Bond</SUMOylationand other PTM identification, including whether modifications occur, the type of modification, the amino acid sites of modification, and the content.
BTP has years of experience in proteomics services, with a comprehensive quality control workflow and experienced technicians capable of handling various samples. We can tailor project plans based on actual research needs, providing you with a worry-free one-stop proteomics service. You only need to inform us of your experimental goals and send the samples; BTP will handle all subsequent aspects of the project. For more proteomics services, feel free to consult us!
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