Methylation modification is one of the most common post-translational modifications of proteins. Methylation mostly occurs on transcription factors and histones, with a small amount occurring on cytoplasmic proteins. Methylation can occur on arginine (R) and lysine (K) residues, including mono-methylation, symmetric/asymmetric di-methylation, and tri-methylation. Arginine methylation can regulate processes such as RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction. Lysine methylation can regulate histone function and participate in the epigenetic regulation of gene transcription. By using specific antibodies against different methylation sites and forms to enrich methylated peptides, combined with LC-MS/MS analysis, large-scale qualitative and quantitative analysis of methylated proteins can be achieved.
Biotech company Thermo Fisher uses the Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoscale chromatography to provide methylation quantitative proteomics analysis services. All you need to do is inform us of your experimental objectives and send your samples. We offer a one-stop service including protein extraction, protein digestion, methylation peptide enrichment, peptide separation, mass spectrometry analysis, raw data analysis, and bioinformatics analysis.
Methylation Quantitative Proteomics Research Service Process
Methylation Quantitative Proteomics Research
Sample Requirements
• Plant roots, xylem, phloem, etc.: More than 5g
• Animal tissues: Wet weight >200mg
• Microorganisms: Wet weight >2g
• Body fluids (saliva, amniotic fluid, cerebrospinal fluid, etc.): More than 10mL
• Serum: More than 500ul
• Urine: More than 50ul
• Protein extracts: Concentration >2mg/mL, total amount not less than 1mg. Samples should not contain components that may affect separation, such as nucleic acids, lipids, and polysaccharides.
Our Advantages
• High throughput: Can identify and quantify up to thousands of proteins at once
• 100% coverage: Use of 2-3 enzymes for protein digestion to ensure complete coverage
• Suitable for analyzing low-abundance methylation-modified proteins
• Advanced detection instruments: Orbitrap Fusion Lumos mass spectrometer with highest resolution and sensitivity
Technical Platform
Ion Chromatography
High-Performance Liquid Chromatography (HPLC)
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS)
About the Samples
1. If you are providing tissue samples, please send them to us with dry ice.
2. If you are providing protein samples, use regular tissue or cell lysis buffer for protein extraction.
3. Sample transportation: Please use sufficient dry ice for transportation and choose a faster delivery method to reduce the possibility of sample degradation during transit.
4. Before the formal experiment, we will test your samples, and the formal experiment will start once the samples pass the test.
Our technicians are experienced and can optimize the experimental plan according to your requirements to ensure high-quality protein methylation analysis results. If you have specific related service needs, please contact us.
Bilingual Project Report
In the technical report, we will provide you with a detailed bilingual (Chinese/English) technical report, including:
1. Experimental Steps (Chinese/English)
2. Relevant Mass Spectrometry Parameters (Chinese/English)
3. Detailed Information of Identified Methylation Sites
4. Mass Spectrometry Images
5. Raw Data
One-Stop Methylation Quantitative Proteomics Service
All you need to do is place an order and send the samples
Biotech one-stop service completes: sample processing - machine analysis - data analysis - project report
