Sphingoglycolipids are widely present in cell membranes and sera. They exist either in a free form or in complex with other proteins. The biological function of sphingoglycolipids is determined by the glycan head groups. Abnormal glycosylation of sphingoglycolipids is closely associated with various cancers, such as breast cancer, lung cancer, and brain tumors. Sphingoglycolipid glycans are also involved in many cellular processes, such as signaling, receptor functions, and cell differentiation. Therefore, detailed analysis of the structure of sphingoglycolipid glycan head groups is of great importance for disease research.
Sphingoglycolipid Glycan Analysis
A comprehensive analysis of sphingoglycolipid glycans requires the use of glycosylase enzymes, which have broad substrate specificity and support high-throughput analysis. Sphingoglycolipid glycan analysis involves the following steps:
1. Extraction and purification of sphingoglycolipids from cell membranes or serum;
2. Release of sphingoglycolipid glycans using glycosylase enzymes;
3. Permethylation of glycans;
4. 2AB-labeling-based UPLC-HILIC-FLD analysis;
5. Analysis of permethylated glycans by time-of-flight mass spectrometry;
If necessary, structural determination of glycans by MS/MS.
One-stop Sphingoglycolipid Glycan Analysis Service
Simply place an order - send your samples
BiotechPack one-stop service completes: sample processing - instrument analysis - data analysis - project report
