Gel and Image Analysis

Gel electrophoresis is an important protein analysis method that includes various techniques such as Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Isoelectric Focusing (IEF). SDS-PAGE is the most commonly used technique in protein analysis, offering a simple method to estimate protein molecular weight, evaluate sample complexity or purity of sample preparation, and monitor products obtained during chromatography or other purification processes. Additionally, ICH Q6B also requires the use of electrophoresis profiles to analyze biopharmaceuticals.

BiotechPack Protein Gel and Image Analysis Services

Gel electrophoresis has been used in protein research for many years, separating proteins based on different physicochemical parameters. BiotechPack offers various protein gel and imaging analysis services.

Advantages of BiotechPack Protein Gel and Image Analysis Services

• Applicable for multiple techniques including molecular weight measurement, impurity characterization, and protein expression studies;
• Suitable for different samples, including body fluids, tissues, blood cells, cell cultures, microorganisms, plants, etc.;
• Various gel size options, including 8×7cm, 23x30cm, 40x30cm, and 60x30cm;
• Experienced technicians providing a wide range of customizable protein gel and image analysis services.

BiotechPack offers the following protein gel analysis services to researchers:

Protein separation based on SDS-PAGE
SDS-PAGE is frequently used in scientific research such as protein expression analysis. It can be utilized to obtain electrophoresis profiles of protein samples and, combined with other mass spectrometry-based protein identification services, analyze or purify samples for protein profiling of complex biological samples.

1D SDS-PAGE and IEF Services
1D SDS-PAGE separates proteins based on different molecular weights and is widely used in protein analysis, including protein sizing, identification, and sample purity analysis.

Two-dimensional Gel Electrophoresis Services
Two-dimensional electrophoresis (2-DE) is a method for separating complex protein mixtures. 2-DE first employs isoelectric focusing (IEF), an electrophoretic technique that separates proteins based on their isoelectric points (pI), to resolve proteins with similar sizes. This is followed by SDS-PAGE for protein separation. BiotechPack provides two-dimensional electrophoresis analysis services. High-resolution two-dimensional electrophoresis can analyze protein species and subtype distribution, host cell protein analysis, quantitative comparison of cell states, etc.

2D Blue Native/SDS-PAGE Complex Analysis Services
In biological membranes, many proteins form complexes. BiotechPack utilizes 2D Blue Native/SDS-PAGE to globally analyze the subunits of these protein complexes. In 2D BN/SDS-PAGE analysis, samples are first separated in one dimension through Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) and then separated in a second dimension via SDS-PAGE.

Protein Immunoblotting and Electrotransfer Services
Protein immunoblotting (Western blot) is another commonly used method for protein analysis, applicable for qualitative and semi-quantitative analysis. In this method, proteins are first separated by two-dimensional electrophoresis (2-DE), and then transferred to a carrier membrane through transfection. Protein immunoblotting can be used for qualitative detection of individual proteins and protein modifications.