16S/18S/ITS full-length sequencing refers to the process of extracting microbial DNA from samples, amplifying the full length of the 16S rDNA, 18S rDNA, or ITS region using universal primers, and then sequencing it. 16S/18S/ITS full-length sequencing can be used to detect microbial diversity.
16S rDNA is present in the genomes of all bacteria and can reflect differences between different bacterial genera. It is currently widely used for the detection and identification of pathogenic bacteria. Full-length sequencing of 16S rDNA can be used to analyze the diversity of bacterial community structures. 18S rDNA is the DNA sequence encoding the small subunit rRNA of eukaryotes, structurally divided into conserved and hypervariable regions. The conserved region reflects phylogenetic relationships between species, while the hypervariable region reflects differences between species. Full-length sequencing of 18S rDNA can be used to analyze eukaryotic microbial community structures. ITS is the internal transcribed spacer sequence between the 18S rDNA and 28S rDNA in eukaryotes. Full-length sequencing of ITS can be used for fungal community structure analysis.
Technical Advantages
Biotech company BiotechPack has experienced technicians who can provide a full range of professional services from experimental design, sample testing, to data analysis;
Clear processes reduce unnecessary sample and time waste, resulting in short delivery times;
Different sample sources use different extraction methods and library sequencing strategies to meet the needs of various environmental research;
Combined with BiotechPack's proteomics, metabolomics, and other analysis platforms, multi-omics integrated analysis can be performed to enhance the quality of publications.
16S/18S/ITS Full-length Sequencing Process
16S/18S/ITS Full-length Sequencing Process
Data Analysis Process
Data Analysis Process
Sample Requirements
Sample Type: Meta samples such as feces, soil, or Meta DNA samples can be tested. Please consult technical personnel for specific requirements.
