With the continuous development of biologics such as proteins and protein complexes and the increasing demand for precise measurement, structural characterization technologies are constantly being updated. When traditional mass spectrometry techniques cannot fully meet the precise measurement needs of complex drugs, native mass spectrometry (Native MS) technology emerges. Native MS is widely used, such as in determining the drug to antibody ratio (DAR) in antibody-drug conjugates (ADCs). ADCs are divided into two types based on the conjugation sites to antibodies: lysine-conjugated and cysteine-conjugated ADCs. Most ADCs are conjugated through lysine with small molecule drugs, and their measurement is usually based on traditional RPLC/MS platforms, where proteins require DAR determination under denaturing conditions. However, these denaturing conditions can cause the separation of light and heavy chains in cysteine-conjugated ADCs, so cysteine-conjugated ADCs can be analyzed using native mass spectrometry.
BaiTaiPaiKe has an advanced native protein mass spectrometry analysis platform - the Exactive Plus EMR mass spectrometer with Protein Deconvolution 3.0 software. Combined with the mass spectrometer's ultra-high resolution, ultra-fast scanning speed, ultra-high mass accuracy, ultra-high sensitivity, and extended mass range, it provides precise analysis for studying the structural, topological, and configurational properties of proteins and protein complexes that retain tertiary and quaternary structures in a state similar to their natural form. It can be used for the analysis of monoclonal antibodies/ADCs, PEGylated proteins, oligomeric protein drugs, glycoforms, and assembled proteins. Additionally, the comprehensive analytical capabilities of the Exactive Plus EMR make it an excellent solution for screening peptides and small molecules.
Native mass spectrometry molecular weight analysis
Features of the Exactive Plus EMR mass spectrometer
• Extended mass range of m/z 350–20,000 allows for the analysis of larger proteins, antibodies, antibody-drug conjugates, glycoforms, and protein assemblies
• Excellent transmission capability for high-mass ions provides stronger signals
• High resolving power with a resolution of up to 140,000 FWHM, enabling the separation of isomers and determination of glycosylation states
• Compatible with Protein Deconvolution software for comprehensive protein characterization analysis
Applications of native mass spectrometry:
• Analysis of ADCs, such as DAR determination
• Analysis of PEGylated or polysaccharide-modified proteins
• Research on assembled protein or protein complex drugs
• Determination of the molecular weight of proteins and protein complexes in their native structural state
• Structural and topological studies of native proteins and protein complexes
Bilingual project report in Chinese/English
In the technical report, BaiTaiPaiKe will provide you with a detailed bilingual technical report in both Chinese and English, which includes:
1. Experimental procedures (in Chinese and English)
2. Relevant mass spectrometry parameters (in Chinese and English)
3. Mass spectrometry images
4. Raw data
5. Detailed information on identified proteins
One-stop Native Mass Spectrometry (Native MS) Service
You only need to place an order and send the samples
BaiTaiPaiKe provides one-stop service: sample processing - instrument analysis - data analysis - project report
