Targeted Metabolomics

Targeted metabolomics is an important part of metabolomics research and an extension and expansion of comprehensive metabolomics studies. Compared to comprehensive metabolomics analysis, targeted metabolomics analysis has several characteristics such as high specificity, high detection sensitivity, and accurate quantification. By enriching and precisely quantifying and qualifying specific metabolites in blood, urine, or other body fluids and tissues, targeted metabolomics can reveal related molecular biological mechanisms in conjunction with other experimental data, and also provide strong support for in-depth research and development of metabolic biomarkers. Targeted metabolomics can be used to: validate hypotheses proposed by untargeted metabolomics experiments; conduct hypothesis-based exploratory experiments to study metabolic models for specific metabolites.

The key factors for the success of targeted metabolomics analysis are accuracy, high throughput, and reliability. Targeted metabolomics identification generally uses selective ion monitoring (SIM) on a GC/MS system or multiple reaction monitoring (MRM) on a triple quadrupole LC/MS system for targeted MS/MS. SIM has higher sensitivity than full scan spectrum acquisition because it extends the acquisition time of selected ions. Since it monitors a very narrow mass window, the mass chromatogram represents detection of very specific masses. In most cases, sensitivity can be improved by 10 times. MRM is the method of choice for mass spectrometry quantification, being more sensitive and specific than SIM. It can generate unique fragment ions, allowing monitoring and quantification of target compounds in very complex matrices.

The general analysis workflow of targeted metabolomics is:
1. Data AcquisitionThe first step is to obtain standards. If they cannot be purchased on the market, they must be custom synthesized. Then optimize the collision energy for product ions to obtain signals, and measure the MRM transition values of metabolites using a triple quadrupole.
2. QuantificationNext, use quantification software to process MRM data and generate reports.
3. Statistical Analysis and InterpretationInput the quantification report into a statistical analysis software package and process the data using statistical tools such as ANOVA.

Targeted Metabolomics Analysis Workflow

Targeted Metabolomics Research Pathway

Targeted Metabolomics Services Developed by BiotechPack

Targeted Metabolomic Pathways	Carbohydrates and Carbohydrate Metabolism
Organic Compounds	Acyl CoAs	Amino Acids and Their Derivatives	Animal Hormones
	Aldehydes	Bioamine	Bile Acids
	Carnitines	Carotenoids	Fatty Acids
	Flavone	Flax-Lignans	Biomarkers
	Nucleotides	Oxysterols	Organic Acids
	Plant Hormone	Resveratrol	Tryptophan Metabolites
	Thiols	Vitamins & Coenzymes
Inorganic Compounds	Metal/Metallomics	Polyphenols

Other Targeted Metabolomics Services by BiotechPacknology:

Sphingolipid Analysis Service
Hexosylceramide
Diacylglycerol
Triglycerides
Short-Chain Fatty Acids
Endocannabinoid Analysis Service
Acyl-CoA Analysis
Cardiolipin Analysis
Oleuropein Analysis
Wax Ester Analysis
Eicosanoid Metabolites Analysis (Urine)
Polysaccharide Analysis Methods and Processes
Hydroxyeicosatetraenoic Acids (HETEs) Analysis
Total Trans Fatty Acids Analysis
ω-6 Fatty Acids Analysis
Prostaglandin Analysis
Ganglioside Analysis
Lipoxygenase Products Analysis
Eicosanoid Analysis
Polypentenol Analysis
Very Long-Chain Fatty Acids Analysis Service
Mycolic Acid Analysis Service
Cholesterol Ester Analysis Service
Sphingolipid Analysis Service
Glycerophospholipid Analysis Service
Free Fatty Acids (FFA) Analysis Service
Leukotriene Analysis Service

About Samples

1. Microbial and Cell Samples: Rapidly quench metabolic activity (quenching) while keeping cells intact.
2. Animal Body Fluids (e.g., urine, blood, tissues, organs, saliva): Rapidly pretreated after sampling, such as adding anticoagulants, preservatives, and immediately freezing (-80°C).
3. Plant Samples: Rapidly freeze after collection (liquid nitrogen), then transfer to -80°C for storage, 200mg per sample.
4. Serum Samples: 500ul per sample (not less than 200ul per sample), avoid repeated freeze-thaw cycles. (Collect blood into centrifuge tubes, let sit for 30 minutes to coagulate. Then centrifuge to take the supernatant into a clean centrifuge tube, centrifuge again for 5 minutes at 8000rpm, aliquot the supernatant into cryotubes, and send frozen at -80°C.)
5. Urine Samples: 1ml per sample, can take more in principle (Directly aliquot urine into centrifuge tubes, 1ml per tube, add one drop (about 10ul) of sodium azide with a mass volume of 1/100 (w/v), send frozen at -80°C).

Chinese/English Data Report

In the final technical report, BiotechPacknology will provide you with a detailed bilingual Chinese/English technical report, including:

1. Experimental Steps (Chinese and English)
2. Relevant Mass Spectrometry Parameters (Chinese and English)
3. Mass Spectrometry Images
4. Raw Data
5. Metabolic Molecule Identification Results

One-Stop Targeted Metabolomics Service

All you need to do is place an order - send the samples
BiotechPack One-Stop Service Completion: Sample Processing - On-machine Analysis - Data Analysis - Project Report

Related Services

Metabolomics
Untargeted Metabolomics Analysis
Lipidomics Research
Untargeted Lipidomics
Targeted Lipidomics
Targeted Proteomics