Protein Purity and Homogeneity Characterization

Protein purity and homogeneity are crucial for protein crystallization and rigorous structural and functional studies. Impure samples can lead to inaccurate results for the entire experiment. Moreover, protein purity and homogeneity are two very important characteristics for further protein research and biopharmaceutical applications. The purity of protein samples limits the application of protein products.

Common contaminants in protein products include culture media, reagents from extraction and purification steps, etc. Besides impurities, protein heterogeneity can result from different intracellular reactions and post-translational modifications. It is important to analyze the purity and homogeneity of proteins before further processing.

Characterization of protein purity and homogeneity using pI labels

 

BTP-Protein Purity and Homogeneity Characterization Services

BiotechPack provides a series of services for characterizing protein purity and homogeneity, detecting contaminants, protein variants, isomers, S-S chain mismatches, truncated protein products, degraded proteins, protein modifications, protein aggregates, and protein precursors.

Purity and Integrity Analysis Methods:SDS-PAGE, capillary electrophoresis, zinc reverse staining, non-glutaraldehyde modified silver staining, MALDI-TOF mass spectrometry.
Homogeneity Analysis:Dynamic light scattering, UV-visible fluorescence spectroscopy, size-exclusion chromatography, static light scattering.

Sample Requirements
We can process samples such as antibodies, antigens, enzymes, growth factors, DNA-binding proteins, blood proteins, membrane proteins, etc. Common samples include:
• Proteins from natural sources
• Recombinant fusion proteins and tagged proteins
• Antibodies of different subtypes
• Membrane proteins

Advantages of BTP-Protein Purity and Homogeneity Characterization

• Advanced Technology Platform:We are equipped with various technology platforms to accurately determine the purity and homogeneity of proteins;
• Accuracy and Reproducibility:We provide accurate and detailed results regarding sample purity and homogeneity, saving time wasted on poor samples;
• Wide Application Range:Purified and homogeneous proteins can be used for research and medical applications, such as the development and quality control of therapeutic proteins.

BiotechPack offers a variety of methods to economically and efficiently characterize samples, providing reliable and detailed data reports. For specific service requirements, please contact our technical staff.