Glycosylation Site Detection
Glycosylation, especially N-glycosylation, is a common post-translational modification that regulates the localization, function, activity, lifespan, and diversity of proteins in organisms. Research on N-glycosylation sites is one of the important prerequisites for understanding the function of glycan chains. In the information related to N-glycosylation, the sites where N-glycosylation occurs are the basis for understanding its glycan function. Besides traditional biochemical methods such as site-directed mutagenesis for studying glycosylation sites, mass spectrometry also plays an important role in the identification of N-glycosylation sites. MALDI TOF MS and nano LC-ESI-MS/MS are commonly used for the identification of N-/O-glycosylation sites in antibodies. Bytway Parker possesses both MALDI TOF MS and nano LC-ESI-MS/MS mass spectrometry technologies, providing efficient and accurate glycosylation identification services for biopharmaceuticals including proteins and antibodies. After receiving samples, we first perform enzymatic digestion on the samples to release the Fc domain and Fab of the antibodies, then separate the glycosylated fragments and confirm the glycosylation sites via MALDI TOF MS or nano LC-ESI-MS/MS.
Glycosylation site analysis of biopharmaceuticals
Glycosylation site identification process
(1) Perform enzymatic digestion on the sample using trypsin or other appropriate proteases;
(2) Select suitable chromatography columns to separate N-glycopeptides, O-glycopeptides, and other peptides;
(3) Perform deglycosylation treatment;
(4) Analyze peptide maps via MALDI-TOF MS, ESI-MS, or MS/MS to identify theoretical glycosylation sites;
(5) Determine each N-/O-glycan site from the peptide maps;
(6) Compare glycosylation sites across different batches of samples.
Chinese/English project report
In the technical report, Bytway Parker will provide you with a detailed bilingual technical report in both Chinese and English, including:
1. Experimental steps (Chinese and English)
2. Relevant mass spectrometry parameters (Chinese and English)
3. Mass spectrometry images
4. Raw data
5. Glycosylation site information of biopharmaceuticals
One-stop service for glycosylation site determination of biopharmaceuticals
You only need to place an order and send the samples
Bytway Parker's one-stop service completes: sample processing - instrumental analysis - data analysis - project report
Related services
Glycan profiling of biopharmaceuticals
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