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N-Glycosylation Site Analysis

N- Glycosylation (N-glycosylation) isEukaryoticIn organismsA type ofCommon form of glycosylation modification,Under the action of glycosyltransferases,Oligosaccharide chainsWithProteinsOnTheSpecificAmino acid residues—AsparagineForm amide bonds between,Thus binding to the protein.N-glycosylation sites are the amino acid sites where N-glycosylation modification occurs,N-glycosylation site analysisThrough protein digestion and mass spectrometry technology,Identify and locate the specific amino acid residues in proteins where N-glycosylation occurs,Analysis results can reveal the structure and function of proteins,Which is crucial for ensuring that biological products function properly.In-depth analysis of N-glycosylation sites is expected to reveal more biological mysteries and guide the development of new drugs.

Bio-Tech Pack BTPCertified by CNAS/ISO9001 dual quality system. The companyBased onStable and efficient liquid chromatography high-resolution mass spectrometry (LC-MS/MS) has developed a series ofGlycosylationCharacterization methods,Providing you with high-quality one-stop N-glycosylation site analysis services, including NGlycan analysis, NGlycosylation site identificationAndComplete analysis process of glycopeptide resolution.Welcome to consult for free to learn more details!

Experimental instruments

• NanoLC: Vanquish NEO

• MS: Orbitrap Fusion Lumos

Case illustration

UtilizingUsing PNGase F in heavy water to release glycans and label18O methodTo performN-glycosylation site analysis. First, based on the theoretical amino acid sequence information of the protein, select the appropriate protease to cleave the protein into peptides. After solvent evaporation, we perform deglycosylation in heavy water. Then, through liquid chromatography-high-resolution mass spectrometry technology, we can determine the peptide segments containing18O-modified N-glycosylation sites, thereby determining the location of N-glycosylation.

Figure2 N-glycosylation site secondary diagram

Note: In the figure, the N-glycosylation modification is on the eighth amino acid of the peptide segment, with a molecular weight change of +2.988, the horizontal axis is the mass-to-charge ratio, and the vertical axis is the signal intensity

Chinese/English project report

In the technical report, Bio-Tech Pack will provide you with a detailed bilingual technical report in both Chinese and English, including:

1. Experimental steps (Chinese and English)
2. Relevant mass spectrometry parameters (Chinese and English)
3. N-glycosylation site analysisDetailed information
4. Mass spectrometry images
5. Raw data

N-glycosylation site analysisOne-stop service

You only need to place the order and send the samples
Bio-Tech Pack's one-stop service completes: sample processing, on-machine analysis, data analysis, and project report