Glycomics Analysis Service

Glycomics refers to the collection, analysis, and application of glycan biological data at the omics level. The profound biological effects of lipid and protein glycosylation make glycomics an important field in life sciences. N-glycan profiling (comprehensive study of all glycans) is a good complement to genomics and proteomics research. Glycomics research at the cellular or organism level can provide a general overview of the total glycosylation patterns of glycomes, glycoproteins, lipids, or other types of biomolecules. Glycomics analysis can be used to compare differences in therapeutic glycoprotein production between two glyco-engineered host strains or to compare data from different stages of cancer, thereby discovering new biomarkers, among other applications.

 

Glycomics Analysis Services

 

Bio-Techne Glycomics Analysis Services

This service can be used to identify the complete N-glycome expressed in plasma/serum, cells, tissues, or organisms. All glycans linked to proteins will be enzymatically digested and hydrolyzed, separated by hydrophilic chromatography, and quantitatively analyzed using the MALDI-TOF MS system.

The general steps of glycomics analysis are as follows:
1. Sample preparation
2. Separation of glycoprotein and/or glycolipid components
3. Hydrolysis of N-, O-, and/or glycosphingolipid glycans
4. Methylation of glycans
5. MALDI-TOF MS analysis of native and methylated glycans
6. Glycan structure determination through MS/MS fragmentation
7. Statistical analysis of potential biomarker glycans

Bio-Techne provides the following protein analysis services:

Glycoprotein Analysis
Glycosylation Quantitative Proteomics Studies
N-Glycan Analysis Services
O-Glycan Analysis Services
N-Glycosylation Site Analysis
O-Glycosylation Site Analysis
N-Glycan Modification and Modification Site Analysis Services
O-Glycan Modification and Modification Site Analysis Services
HILIC-UHPLC Analysis of N-Glycan Bonds

Related Services

Methylation Quantitative Proteomics Studies
Post-Translational Modification Proteomics Analysis
Quantitative Proteomics Analysis
Targeted Proteomics
4D Proteomics