Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry for the identification of protein samples that may produce fragments through ionization methods. Compared to another soft ionization technique, electrospray ionization (ESI), MALDI generates fewer multiply charged ions and allows for full-spectrum analysis of protein samples without the need for additional deconvolution steps. However, for proteins with larger molecular weights, the difference between average mass and accurate mass is significant. Therefore, for proteins with molecular weights greater than 25 kDa, high-resolution mass spectrometry identification strategies are recommended. BiotechPack provides MALDI-TOF-MS analysis suitable for complete mass determination of proteins with molecular weights below 25 kDa.
MALDI TOF Molecular Weight Determination
Sample Requirements
Regarding samples: Samples should be dissolved in volatile solvents with low content of salts and detergents.
Sample Transport: Lyophilized protein samples should be transported frozen or at 4°C.
Advantages of MALDI-TOF MS for Molecular Weight Identification:
1. MS/MS spectra can be obtained, providing peptide amino acid composition information
2. High resolution: ~20000
3. High sensitivity: ~fmol, amol
4. Fast analysis speed, high throughput
5. High mass accuracy: ~20 ppm
6. Large molecular weight measurement range: ~200000 Da
7. Some tolerance to impurities such as salts in samples
Case Analysis:
Since MALDI employs matrix-assisted laser desorption as a soft ionization method, it can analyze whether there are oligomers in protein or peptide samples during molecular weight determination. The following is the actual result of determining the molecular weight of a protein drug, showing that there are small amounts of dimer and trimer structures in the protein sample.
Bilingual Project Report
In the technical report, BiotechPack will provide you with a detailed bilingual version in both Chinese and English, including:
1. Experimental Steps (Chinese and English)
2. Relevant Mass Spectrometry Parameters (Chinese and English)
3. Mass Spectrometry Images
4. Raw Data
5. Protein/Peptide Molecular Weight Information
One-stop MALDI-TOF Molecular Weight Determination Service
You only need to place an order and send the sample
BiotechPack completes the one-stop service: sample processing - machine analysis - data analysis - project report
