High-Throughput Gene Knockout Service
"Knocking out" genes in mammals is an important method for studying gene function or their potential as drug targets. It is widely used in research into neurodegenerative diseases such as Alzheimer's disease, cancer, cardiovascular diseases, inflammation, or metabolic disorders. CRISPR/Cas9 is a revolutionary gene editing technology that can precisely modify genome sequences. CRISPR is an immune system found in bacteria and archaea that can recognize and cut the genomes of invading viruses. By introducing the Cas9 protein and corresponding guide RNA, CRISPR/Cas9 technology can precisely locate and cut specific DNA sequences, enabling gene knockout, point mutations, and gene insertion. Gene editing mediated by CRISPR/Cas9 has enormous clinical application potential. As this technology matures, it plays an increasingly important role in drug target discovery and validation, gene function research, gene therapy, cancer immunotherapy, and many other fields.
Neil, C.; et al. ACS Synthetic Biology. 2022.
CRISPR/Cas9 Gene Knockout Technology
To meet the needs of basic research and pharmaceutical development, there is an urgent demand in the scientific community for knockout cells for a series of target genes/whole genomes. BTP (Biotech) uses an optimized CRISPR/Cas9 system to provide you with high-throughput gene knockout services. Our human whole-genome gene knockout array library covers the entire human genome, and the whole-genome array knockout library has been fully validated in primary human cells with high-content screening, achieving over 70% knockout efficiency for more than 80% of targets.
BTP is committed to providing you with the simplest and most user-friendly high-throughput CRISPR gene knockout services, including viral packaging, plasmid preparation, Cas9 concentration testing, etc. We solve key questions such as which cells to knockout, whether it can be knocked out, how to knockout, and the efficiency of knockout. We can perform single/multiple gene knockouts, frame-shift mutations, and large sequence deletions. You only need to provide the gene name and target cells, and BTP Biotech will handle all subsequent projects, including project evaluation (whether the target gene is expressed in the target cells, whether it is an essential gene), KO experiments, KO cell validation, KO cell cryopreservation, and delivery of live KO cells.
Service Advantages
1. Strong Sample Applicability
Applicable to human and mouse cell lines, primary cells, immune cells, and iPS cells, etc.
2. Gene Necessity and Expression Level Analysis
Before conducting gene knockout experiments, we analyze the necessity and expression levels of genes in different cells based on bioinformatics and proteomics data to ensure the feasibility of the experiment and reduce experimental risks.
3. No Need for De Novo gRNA Design and Verification
We have completed the validation of the entire human genome gRNA, and drug-related targets have also completed PCR sequencing validation. There is no need for pre-experiment gRNA validation for customer targets, significantly shortening the project cycle.
4. High-Throughput and High Efficiency
We use an optimized CRISPR/Cas9 system for high-throughput gene knockout, with multiple sgRNA/target gene designs ensuring high knockout efficiency. More than 80% of targets achieve over 70% knockout efficiency, and a high-efficiency KO pool can be provided within a short time (within 10 days), meeting most application scenarios.
5. Dual Verification of Knockout Efficiency
After gene knockout, we use Sanger sequencing and advanced proteomics techniques, the preferred methods for antibody KO validation in WB experiments, to perform dual verification of knockout efficiency, ensuring double assurance of data authenticity.
Application Cases
1. Gene Therapy
Dever DP, et al. Nature. 2016.
2. Immune Cell Engineering
Roth TL, et al. Nature. 2018.
3. Gene Function Research
Gordon DE, et al. Science. 2020.
4. Drug Target Screening
Morgens DW, et al. Nat Biotechnol. 2016.
Frequently Asked Questions:
Question 1: What are the advantages of high-throughput gene knockout technology compared to traditional gene knockout techniques?
BiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services ProviderCompared to traditional gene knockout techniques such as lentiviral packaging and plasmid methods, high-throughput gene knockout technology has the following advantages:
1) Editing Throughput
- Traditional gene knockout techniques: Low throughput, usually taking 2-3 months to construct dozens of knockout cell lines.
- High-throughput gene knockout technology: High throughput, usually obtaining hundreds of knockout cell lines in 1-2 months.
2) Editing Efficiency
- Traditional gene knockout techniques: Knockout efficiency ranges from 30-90% and is inconsistent.
- High-throughput gene knockout technology: Very high knockout efficiency, reaching 70-100%.
3) KO Cell Inventory
- Traditional gene knockout techniques: KO cell inventory is less than 3000+.
- High-throughput gene knockout technology: Covers the entire human genome, with KO cell inventory exceeding 19000+.
Related Services
High-throughput gene knockout technology for drug screening and target identification
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