Protein Isoelectric Point Determination
Proteins are amphoteric molecules that contain both positively and negatively charged functional groups, and their overall charge is usually determined by the pH of their surrounding environment. When the external pH causes the acidic dissociation and basic dissociation of the protein molecule to be equal, that is, the positive and negative charges of the molecule cancel each other out, the pH at this point is called the isoelectric point (pI) of the protein. When pH=pI, the total surface charge of the protein is zero, and the protein solubility is minimal. The isoelectric point of a specific protein is fixed and related to the composition and conformation of the protein, so precise determination of the isoelectric point is often used as an effective means of protein identification. In addition, the protein isoelectric point is also an important indicator in many common biochemical analyses and proteomics experiments, such as two-dimensional gel electrophoresis (2D-PAGE), X-ray crystallography, and liquid chromatography-mass spectrometry.
Biotyech Labs, based on capillary isoelectric focusing (cIEF), can achieve precise determination of the isoelectric point of any protein. Compared to the traditional slab method, cIEF has the advantages of being efficient in a short time, highly accurate, and requiring a small sample amount. This technique is suitable for various amino acids, peptides, recombinant proteins, enzymes, monoclonal antibodies, etc., and has been widely used in clinical analysis.
Santhi, O. K. et al. Indo Am. J. P. Sci. 2021.
Methods for Determining Protein Isoelectric Points
Sample Requirements:
Protein sample concentration >0.5 mg/mL, purity >90%, minimum amount not less than 50 pmol
Chinese/English Project Report
In the technical report, Biotyech Labs will provide you with a detailed bilingual technical report in both Chinese and English, which includes:
1. Experimental steps (Chinese and English)
2. Relevant data (Chinese and English)
Related Services
Protein Molecular Weight Determination
Protein Structure Identification
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