Eukaryotic transcriptome sequencing can be categorized into reference-based and de novo approaches. De novo sequencing refers to the sequencing of an eukaryotic transcriptome without a reference genome. After obtaining the raw data from eukaryotic de novo transcriptome sequencing, quality control is performed to assemble unigenes, which then serve as reference sequences for subsequent analyses, including functional annotation, SNP, and SSR marker development. Multiple samples can also be analyzed for differential gene expression and functional enrichment of differentially expressed genes, aiding in the discovery of functional genes and providing direction for further research.
Bitepike Biotech's transcriptome sequencing services are based on the Illumina high-throughput sequencing platform, capable of detecting the overall transcriptional level of any organism at the single nucleotide level. While analyzing the structure and expression level of transcripts, it can also discover novel and rare transcripts, accurately identifying alternative splicing sites and coding sequence single nucleotide polymorphisms (cSNP), thus providing the most comprehensive transcriptome information.
Eukaryotic De Novo Transcriptome Sequencing Experimental Process
Eukaryotic De Novo Transcriptome Sequencing Experimental Process
Service Advantages
Experienced technicians provide a complete range of professional services from experimental design, sample testing, to data analysis;
Clear procedures to minimize unnecessary sample and time waste, with short delivery times;
Bitepike Biotech has an independent bioinformatics analysis platform, offering advanced custom analyses in addition to routine analyses;
Bitepike's proteomics, metabolomics, and other multi-omics analysis platforms enable integrated multi-omics analysis, enhancing publication quality.
