The transcriptome is a collection of the complete set of transcripts within a cell under specific developmental stages or physiological conditions. The main purpose of transcriptome sequencing is to classify all transcripts, including mRNAs, non-coding RNAs, and small RNAs; determine the transcription structure of genes based on gene start sites, 5' and 3' ends, splicing patterns, and other post-transcriptional modifications; and quantify the changes in the expression levels of each transcript during development and under different conditions. Understanding the transcriptome is essential for interpreting the functional components of the genome, uncovering the molecular composition of cells and tissues, and understanding development and disease. It has been widely applied in basic research, clinical diagnostics, and drug development.
Advantages of transcriptome sequencing technology:
Biteco Biological Technology's transcriptome sequencing service is based on the Illumina high-throughput sequencing platform and can detect the overall transcription level of any species at the single-nucleotide level. While analyzing the structure and expression levels of transcripts, it can also discover unknown and rare transcripts and accurately identify alternative splicing sites and coding sequence single nucleotide polymorphisms (cSNPs), providing the most comprehensive transcriptome information. Compared to traditional chip hybridization platforms, transcriptome sequencing can detect the entire transcription level of any species without the need to pre-design known sequence probes, offering more accurate digital signals, higher detection throughput, and a broader detection range. Transcriptome sequencing analysis is a powerful tool for in-depth study of transcriptome complexity.
Transcriptome sequencing experimental procedure:
Transcriptome sequencing experimental procedure
Transcriptome sequencing analysis procedure:
Transcriptome sequencing analysis procedure
Advantages of transcriptome sequencing services:
Experienced technicians provide a full range of professional services from experimental design, sample testing, to data analysis.
Clear processes minimize unnecessary sample and time waste, with short delivery times.
Biteco Biological Technology has an independent bioinformatics analysis platform, offering not only conventional analyses but also advanced customized analyses.
Biteco has platforms for proteomics, metabolomics, etc., enabling multi-omics integration analyses to enhance the quality of publications.
