Prokaryotic Transcriptome Sequencing

The transcriptome is the complete set of mRNA within a cell at a specific developmental stage or physiological condition. Transcriptome sequencing is widely used as a technical means to study the transcriptome in biological science research. Analyzing the transcriptome information of biological samples helps to study the gene transcription and transcriptional regulation in cells. In prokaryotes, the vast majority of RNA is rRNA, with mRNA accounting for only 1% to 5% of the total RNA. Therefore, to sequence the transcriptome of prokaryotes, mRNA must be purified. Moreover, prokaryotic mRNA does not have a poly A tail like eukaryotic mRNA, so it cannot be directly purified using oligo T. Generally, during prokaryotic transcriptome sequencing, a method to remove rRNA is used to construct the library. BiotechPack employs effective methods to remove rRNA from different prokaryotes, ensuring the quality of transcriptome sequencing.

Technical Advantages

BiotechPack's transcriptome sequencing service, based on the Illumina high-throughput sequencing platform, can detect the overall transcription levels of any species at the single nucleotide level. While analyzing the structure and expression levels of transcripts, it can also discover unknown and rare transcripts, accurately identify alternative splicing sites, and coding sequence single nucleotide polymorphisms (cSNPs), thereby providing the most comprehensive transcriptome information.

Prokaryotic Transcriptome Sequencing Workflow

Prokaryotic Transcriptome Sequencing Workflow

Service Advantages

Experienced technicians can provide a full range of professional services from experimental design, sample testing, to data analysis.
Clear workflow, reducing unnecessary sample and time waste, with a short delivery time.
BiotechPack has its own bioinformatics analysis platform, offering not only routine analysis but also advanced custom analysis.
BiotechPack's proteomics, metabolomics, and other multi-omics analysis platforms can perform integrated multi-omics analysis to enhance the quality of publications.

BiotechPack Transcriptome Sequencing Case Studies

BiotechPack Transcriptome Sequencing Case Studies

Related Services

Transcriptome Sequencing
Metatranscriptome Sequencing
Eukaryotic Reference-Free Transcriptome Sequencing
Eukaryotic Reference-Based Transcriptome Sequencing