Transcriptome sequencing involves sequencing the complete set of mRNA within cells at specific developmental stages or under physiological conditions. It aids in studying gene expression levels and gene structures at a comprehensive level, revealing molecular mechanisms in specific biological processes. Transcriptome sequencing has been widely applied in basic research, clinical diagnostics, drug development, and molecular breeding, among other fields. Eukaryotic reference-based transcriptome sequencing refers to the sequencing of transcriptomes of eukaryotes or their tissues and cells with a reference genome. By aligning with the reference genome, it is possible to verify the sequencing saturation and gene coverage of transcriptome sequencing and obtain information on gene structure, gene expression differences, and alternative splicing.
BaiTai Park Biotech's transcriptome sequencing services are based on the Illumina high-throughput sequencing platform, capable of detecting the overall transcription level of any species at the single-nucleotide level. While analyzing the structure and expression levels of transcripts, it can also discover unknown and rare transcripts and accurately identify alternative splicing sites and coding sequence single nucleotide polymorphisms (cSNPs), thus providing the most comprehensive transcriptome information.
Eukaryotic Reference-Based Transcriptome Sequencing Data Analysis Workflow
Eukaryotic Reference-Based Transcriptome Sequencing Data Analysis Workflow
Service Advantages
Experienced technical staff can provide a full range of professional services from experimental design, sample testing, to data analysis;
Clear workflow reduces unnecessary sample and time waste, with a short delivery time;
BaiTai Park Biotech has an independent bioinformatics analysis platform, and can offer advanced custom analyses in addition to conventional analyses;
BaiTai Park's proteomics, metabolomics, and other multi-omics analysis platforms allow for integrated multi-omics analyses, enhancing the quality of publications.
Transcriptome Sequencing Sample Requirements
Cells (≥5×10^6), fresh plant tissue (≥500 mg), animal tissue (≥300 mg), blood (≥2 ml), FFPE samples (≥10 sections not processed with HE staining);
RNA samples (nucleic acid quantity ≥3 μg, 260/280 ≥2.0, RIN value ≥7, 28S/18S ≥1.0, concentration ≥50 ng/μl, volume ≥10 μl).
BaiTai Park Biotech Transcriptome Sequencing Case Studies
BaiTai Park Biotech Transcriptome Sequencing Case Studies
