mRNA 3’ poly(A) tail analysis
In eukaryotes, the 3' end of mRNA is usually appended with a string of purine nucleotides known as the poly(A) tail, which is a variable length chain of adenosine nucleotides. This poly(A) tail plays a crucial role in the stability, transport, and translation of mRNA. mRNA 3' poly(A) tail analysis is a common biological technique used to study the phenomenon of 3' end tailing in mRNA molecules. It has significant applications in the fields of gene expression regulation, cell development, and disease research. mRNA 3' poly(A) tail analysis helps us understand the processing, stability, and transport mechanisms of mRNA and the biological processes related to post-transcriptional regulation. Moreover, this technique plays an important role in drug development, disease diagnosis, and treatment, aiding in the in-depth understanding of the molecular mechanisms of diseases and providing valuable information for designing therapeutic strategies.
mRNA molecules with a 3' end poly(A) tail
mRNA 3' poly(A) tail analysis based on endonuclease and LC-MS technology utilizes RNase T1 digestion and oligo dT magnetic bead purification to obtain the poly(A) tail, followed by qualitative and quantitative analysis of the poly(A) tail using liquid chromatography-mass spectrometry (LC-MS). This method is also one of the more commonly used poly(A) tail analysis methods in current mRNA in vitro preparation processes. BTP Biology Technology (BTP) is certified by dual quality systems CNAS/ISO9001. The company, using Thermo's Obitrap Fusion Lumos mass spectrometer combined with Nano-LC nano-liquid chromatography technology, has established a high-precision mRNA 3' poly(A) tail analysis platform, providing you with a one-stop service for mRNA 3' poly(A) tail analysis, accurately analyzing the length of the poly(A) tail. Feel free to inquire for more details!
Bilingual project report in Chinese/English
In the technical report, BTP will provide you with a detailed bilingual technical report in both Chinese and English, which includes:
1. Experimental procedures (Chinese and English)
2. Relevant mass spectrometry parameters (Chinese and English)
3. Detailed information on mRNA 3' poly(A) tail analysis
4. Mass spectrometry images
5. Raw data
One-stop service for mRNA 3' poly(A) tail analysis
You only need to place an order and send your samples
BTP's one-stop service includes: sample processing, on-machine analysis, data analysis, and project report
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