mRNA 5' Cap Rate Analysis

mRNA 5' capping refers to the process of adding a 7-methylguanosine (m7G) cap to the 5' end of newly synthesized mRNA molecules. It is one of the key steps in eukaryotic gene expression, crucial for the stability, export, translation, and degradation of mRNA. mRNA capping efficiency analysis evaluates whether the 5' end of mRNA molecules is modified with an m7G cap. This analysis plays an important role not only in fundamental biological research but also has potential applications in disease diagnosis and treatment. For example, many diseases, including certain types of cancer, are associated with abnormalities in the mRNA capping process. mRNA capping is also a critical component of modern mRNA vaccines, such as the COVID-19 vaccines.

mRNA molecules with 5' ends modified by m7G caps

The m7G-modified 5' cap increases the mass of RNA molecules, which can be detected by mass spectrometry. In mass spectrometry, RNA molecules with 5' caps show larger m/z value peaks compared to uncapped RNA molecules. By measuring the relative intensities of these two peaks, the 5' capping efficiency can be calculated. Bio-Pharm Technology (BTP) is certified by CNAS/ISO9001 dual quality systems. The company uses Thermo's Obitrap Fusion Lumos mass spectrometer combined with Nano-LC nano-chromatography technology to establish an mRNA 5' capping efficiency analysis platform, providing one-stop mRNA 5' capping efficiency analysis services. You just need to inform us of your experimental objectives and send the samples, and BTP will handle all subsequent project tasks. For more details, feel free to contact us!

Bilingual project report in Chinese/English

In the technical report, BTP provides you with a detailed bilingual (Chinese and English) technical report, which includes:

1. Experimental procedures (Chinese and English)
2. Relevant mass spectrometry parameters (Chinese and English)
3. Detailed information on mRNA capping efficiency analysis
4. Mass spectrometry images
5. Raw data

mRNA capping efficiency analysisOne-stop service

You only need to place an order and send the samples
BTP's one-stop service includes: sample processing, machine analysis, data analysis, and project report

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