Top Down Protein Sequencing

In top-down proteomics analysis, intact protein molecule ions generated by ESI or MALDI are introduced into a mass analyzer and undergo gas-phase fragmentation. The top-down approach allows for direct observation of sequence truncations at the C-terminus and N-terminus and distinguishes isomers through different sequences.

Protein sequencing analysis service based on the top-down approach

Compared to the traditional Edman degradation method, BiotechPack offers protein sequencing analysis services based on the top-down approach, using MALDI ISD mass spectrometry technology to provide N-terminal and C-terminal sequencing services. Sequencing can be performed even when the termini are modified, including N-terminal acetylation, N-terminal pyroglutamate, sequence truncations, signal sequences, or blockage. This analysis can also be used to characterize protein degradation and enzymatic cleavage.

Top-down sequence analysis can confirm 20 to 80 residues at the N-terminus or C-terminus of the intact protein.