Good, active, and effective antibodies represent a multi-billion dollar industry. Simply possessing hybridoma cells is far from sufficient, as storage accidents, genetic drift, or contamination can threaten antibody safety. Therefore, when you obtain such an antibody, a more effective approach is to perform sequence analysis on the antibody's sequence, allowing for recombinant production at any time. Alternatively, if you have an effective antibody and wish to protect its intellectual property or expand its production, obtaining precise sequence information is a necessary process. Conducting sequence analysis on antibodies is the starting point for protecting your antibodies and initiating antibody engineering projects, such as species, isotype and subtype switching, and even antibody humanization.
Biotyech-Peak Biotechnology's Mass Spectrometry-Based Monoclonal Antibody Sample Sequence Analysis
It's too late to sequence the hybridoma if the cell line has been lost or is unavailable. With the continuous advancement of mass spectrometry technology, as long as there is still a sufficient amount of antibody, sequencing of the antibody sample can be achieved. Monoclonal antibodies play a key role in drug development and diagnostic reagent development. The primary structure of the antibody molecule itself, particularly the amino acid sequence of its CDR region, is the core of its biological function, and accurate and rapid analysis of its complete sequence is of great importance.
Biotyech-Peak Biotechnology, utilizing the world's highest resolution mass spectrometer, Orbitrap Fusion Lumos, combined with extensive bioinformatics analysis experience, has established a new generation antibody sequencing platform capable of rapid and accurate analysis of antibody primary structures. This platform is suitable for sequence analysis of different antibody subtypes (such as IgG and IgM) and different types of antibodies (fluorescently labeled antibodies, immobilized antibodies, antibodies from various species). Biotyech-Peak Biotechnology's antibody sequencing service can analyze the full-length sequence, variable region amino acid sequence, providing customers with complete sequence information of the antibody's light and heavy chains.
*Biotyech-Peak Biotechnology's one-stop antibody sequencing service ensures you obtain antibody sequence information and a complete sequencing report, achieving full sequence coverage of the antibody, including the complete sequences of the heavy and light chains.All antibody sequencing services by Biotyech-Peak Biotechnology are independently completed by our laboratory, ensuring the highest level of intellectual property security for you.
Antibody Sequencing Diagram
Advantages of Biotyech-Peak Biotechnology's Antibody Sequencing:
Full Sequence Analysis of Antibodies:Optimized high-resolution mass spectrometry workflows and top-notch de novo sequencing software ensure the entire antibody sequence is analyzed from N-terminus to C-terminus;
Unmatched Accuracy:The identification of each amino acid in the sequence is deduced from signals in multiple MS/MS mass spectrometry data from multiple peptides;
Broad Applicability:This technology is applicable to all antibody types (IgG, IgA, IgM, IgY, Fab, ScFv, etc.) and species (human, mouse, rat, rabbit, hamster, etc.). Biotyech-Peak can also sequence conjugated and contaminated proteins and antibodies with two different light chains. Antibody sequence analysis of murine and human antibodies ensures the activity of synthetic antibodies;
Pursuit of Perfection:Biotyech-Peak Biotechnology's antibody sequencing service is committed to mass spectrometry-based antibody sequence analysis. The workflow and bioinformatics analysis process have been optimized to the greatest extent, providing the highest quality service even for the most challenging antibodies to sequence;
High Throughput:Whether you need to sequence just one antibody or hundreds, Biotyech-Peak Biotechnology's antibody sequencing service can accommodate.
About Samples:Monoclonal antibody, sample amount >50-100 ug, if your sample amount is <50 ug, please contact us.
Determination Period:Typically around 3 weeks after receiving the sample.
