Protein Full Sequence Determination

The analysis process of recombinant protein expression and purification products, especially the development and process establishment of recombinant protein or antibody agents, requires confirmation of the full length of the protein. Baitaitek Company utilizes its existing high-resolution mass spectrometry technology platform to develop a mass spectrometry-based protein full sequence technology, achieving 100% coverage of the target protein sequence being measured. This analysis can be used to confirm whether the recombinant protein is fully expressed and to detect any cleavage during the recombinant protein expression process.

In the protein sample identification process, only Trypsin is generally used for enzymatic digestion of the protein, with coverage of identified peptides at about 60%. To obtain the complete sequence information of the target protein, Baitaitek Company selects six commonly used proteases (Trypsin, Chymotrypsin, Asp-N, Glu-C, Lys-C, and Lys-N) for enzymatic digestion and identification of the target protein. By obtaining fragmented peptide segments and assembling them, the 100% determination of the protein sequence is completed.

Protein full sequence determination flowchart

Applications

• Full amino acid sequence analysis of biological products such as proteins, peptides, antibodies, and vaccines

• Analysis of protein post-translational modifications and chemical modifications

• Quality analysis of in vitro synthesized proteins and recombinant protein expression

About Samples

1. Both in-gel and solution samples can be used for the aforementioned sequencing
2. Required amount of target protein sample: 10-20ug
3. Higher sample purity is preferable

Research Case

In this project, a well-known domestic pharmaceutical company sent us an antibody drug under development for complete antibody sequence identification. By selecting multiple proteases and using high-resolution mass spectrometry analysis technology, the results obtained are as follows:

Protein full sequence determination research case

Chinese/English Project Report

In the technical report, Baitaitek will provide you with a detailed bilingual Chinese-English technical report, including:

1. Experimental steps (Chinese and English)
2. Relevant mass spectrometry parameters (Chinese and English)
3. Mass spectrometry images
4. Raw data
5. Detailed information on the protein full sequence

One-stop service for protein full sequence sequencing

You only need to place an order and send the samples

Baitaitek's one-stop service completes: sample processing, machine analysis, data analysis, project report