The analysis process of recombinant protein expression and purification products, especially the development and process establishment of recombinant protein products, requires confirmation of the N-terminus and C-terminus sequences of the protein. PEPROTEK has developed mass spectrometry-based protein N-terminus and C-terminus sequencing technology using its existing mass spectrometry technology platform. This technology allows for the determination of both N-terminus and C-terminus sequences in a single experiment. The mass spectrometry-based N-terminus sequencing technology can achieve sequence determination of N-terminally blocked and PEGylated proteins, complementing Edman sequencing. This analysis can be used to confirm whether the recombinant protein is fully expressed, to detect any breakage during the expression process, and to determine if there are any modifications to the N-terminus and C-terminus sequences of the recombinant protein.
Basic principles of protein N-terminus and C-terminus sequencing based on Peptide Mapping
After obtaining the target protein sequence, we first analyze the customer's target protein sequence, select specific proteases for digestion, obtain suitable lengths of protein N-terminus and C-terminus peptides, and then conduct mass spectrometry analysis to confirm the target protein's N-terminus and C-terminus sequence information.
Protein N-terminus and C-terminus sequencing process
About samples
1. Both in-gel samples and solution samples can be used for the aforementioned N-terminus and C-terminus sequencing.
2. Required amount of target protein sample: 5-10 µg
3. The higher the sample purity, the better
Research case studies
As shown in the figure below, C-terminus sequencing can provide detailed information about the identified amino acids.
Case studies of protein N-terminus and C-terminus sequencing based on Peptide Mapping
Project reports in Chinese/English
In the technical report, PEPROTEK provides you with a detailed bilingual technical report in both Chinese and English, including:
1. Experimental steps (in both Chinese and English)
2. Relevant mass spectrometry parameters (in both Chinese and English)
3. Detailed information on protein N-terminus and C-terminus sequences
4. Mass spectrometry images
5. Raw data
One-stop service for protein N/C-terminus sequencing
You only need to place an order and send the samples
PEPROTEK provides a one-stop service: sample processing - on-machine analysis - data analysis - project report
