Hydroxy-eicosatetraenoic acid (HETEs) Analysis

It is known that arachidonic acid can be oxidized through several enzymatic pathways, including prostaglandin H2 synthase, 5-lipoxygenase, and cytochrome P450-dependent epoxygenase reactions. These enzymatic pathways require free arachidonic acid as a substrate and produce a variety of biologically active lipid mediators, including prostaglandins, thromboxanes, leukotrienes, and epoxy-eicosatrienoic acids. The major arachidonic acid metabolites in the blood and blood vessels are hydroxyeicosatetraenoic acids (HETEs). HETEs are associated with physiological responses such as aggregation, cell migration, and cell proliferation. After high-performance liquid chromatography separation, GC/selected ion monitoring is usually used for sensitive and specific analysis of HETEs.

Analysis of Hydroxyeicosatetraenoic Acids (HETEs)

Bio-Techne offers reliable, rapid, and cost-effective analysis services for hydroxyeicosatetraenoic acids (HETEs) and their isomers based on a high-stability, reproducible, and highly sensitive system for separation, characterization, identification, and quantification analysis combined with LC-MS/MS.

Bio-Techne can provide analysis of the following HETEs

HETEs (5-, 8-, 9-, 11-, 12-, 15-, 20-HETE), HEPEs, and HDoHEs

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