Oligonucleotide Purity Analysis
Oligonucleotides, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), have broad applications in various fields such as gene therapy, biological probes, and diagnostic testing. For example, oligonucleotides canDNAbe introduced into immune cells to genetically modify them to express chimeric antigen receptor proteins for cell-based immunotherapy. Oligonucleotides synthesized using solid-phase synthesis and other techniques may introduce trace impurities at various steps of the synthesis cycle. Therefore, analyzing and testing the purity of oligonucleotides is particularly important to ensure efficacy, accuracy, safety, and bioactivity during the production and research process.
Kanavarioti, A. et al. Sci Rep. 2019.
Ion-Pair Reverse-Phase High-Performance Liquid Chromatography (IP RP HPLC) for Oligonucleotide Purity Analysis
SDS-PAGE is a traditional purification method that can be used to separate oligonucleotides based on size. However, if the oligonucleotides are of similar size and certain modifications are even inseparable, higher-resolution high-performance liquid chromatography is required. The most popular oligonucleotide purification technique is Ion-Pair Reverse-Phase High-Performance Liquid Chromatography (IP RP HPLC). A key advantage of IP RP HPLC is that it can be directly coupled with mass spectrometry for detailed mass characterization of oligonucleotides. BioTech Pack (BTP) has developed and validated an oligonucleotide purity analysis method based on high-resolution liquid chromatography platforms. This method can select appropriate column lengths, mobile phase flow rates, separation temperatures, and ion-pair reagent buffer compositions according to different oligonucleotide samples to identify impurities such as n-1 short chains, n+1 long polymers, incomplete sulfation, and phosphodiester by-products caused by failed reactions. For more oligonucleotide services, feel free to inquire for free!
About the Samples
Identifiable sample types include but are not limited to:
- Synthetic Oligonucleotides: such as PCR primers, probes, etc.;
- Naturally Sourced Oligonucleotides: such as oligonucleotides isolated from RNA or DNA;
- Oligonucleotide Drugs: such as those used in gene therapy, antiviral treatment, and cancer treatment.
Bilingual Project Reports
In the technical report, BioTech Pack will provide you with a detailed bilingual (Chinese and English) technical report, including:
1. Experimental Steps (in Chinese and English)
2. Relevant Chromatographic Parameters (in Chinese and English)
3. Detailed Information on Oligonucleotide Purity Analysis
4. Chromatographic Images
5. Raw Data
Oligonucleotide Purity AnalysisBiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services Provider
You only need to place an order and send the samples
BioTech Pack will complete the one-stop service: Sample Processing - On-Machine Analysis - Data Analysis - Project Report
Related Services
Oligonucleotide Sequence Analysis
Oligonucleotide Molecular Weight Analysis
Nucleotide and Related Substance Analysis
How to order?
