HCP Antibody Coverage Analysis
HCP antibody coverage refers to the proportion of specific host cell proteins (HCPs) that can be detected by antibodies during pharmaceutical production. HCPs are a type of protein impurity that inevitably remains in the final product during the production of biological products, especially when using host cells (such as E. coli, mammalian cells, etc.) to produce recombinant proteins or monoclonal antibodies. These HCPs may affect the safety and efficacy of the drug, so they must be removed or reduced to an acceptable level through specific purification steps.
HCP antibody coverage analysis is based on the principle of specific binding between antigens and antibodies. By combining HCPs in the sample with specific HCP antibodies, and then using other methods such as ELISA, 2D-DIGE, etc., to measure the amount of the complexes formed. By comparing with known concentrations of standards, the content of HCPs in the sample is determined. This process helps us understand how many different kinds of HCPs are recognized and detected by the antibodies, thereby evaluating the coverage of these antibodies.
HCP Antibody Coverage Analysis Based on 2D-DIGE
As the production scale and complexity of biopharmaceutical products increase, HCP antibody coverage analysis has become a crucial step in the quality control of biopharmaceutical products. Moreover, by assessing the purity and quality consistency of products, this method has extensive application value in the biopharmaceutical industry. Bio-Tek (BTP) has passed the dual quality system certification of CNAS/ISO9001; the company has established four HCP antibody coverage analysis technology platforms: 2D-WB, 2D-DIGE, AAE-2D, and AAE-nanoLC-MS/MS, ensuring the accuracy of HCP antibody efficacy analysis while meeting various research needs. Free consultation is welcome to learn more details!
Bilingual Project Report (Chinese/English)
In the technical report, Bio-Tek will provide you with a detailed bilingual (Chinese and English) technical report, including:
1. Experimental procedures (Chinese and English)
2. Relevant experimental parameters (Chinese and English)
3. Detailed information on HCP antibody coverage analysis
4. Relevant images
5. Raw data
HCP Antibody Coverage AnalysisOne-Stop Service
You only need to place an order and send the sample
Bio-Tek's one-stop service completes: sample processing - instrumental analysis - data analysis - project report
Related Services
Host Cell Protein (HCP) Residue Analysis Services
2D-DIGE Quantitative Proteomics
2D-DIGE Antibody Coverage Analysis
How to order?
