2D-DIGE Antibody Coverage Analysis
Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), also known as two-dimensional fluorescence differential gel electrophoresis, is a proteomics technique used for qualitative and quantitative comparison of protein expression changes in different samples (such as diseased and healthy tissues). It plays a crucial role in the production of biological products, especially in HCP antibody coverage analysis. HCP antibody coverage analysis is typically used to assess the sensitivity and specificity of antibodies for detecting HCP. The 'coverage' here usually refers to the proportion of HCP types that can be detected by the antibody out of all HCP types. A high-coverage antibody means it can detect more types of HCP, thus providing a more comprehensive assessment of impurity levels in the production process.
In 2D-DIGE antibody coverage analysis, proteins from different samples are labeled with different fluorescent dyes and then mixed for two-dimensional gel electrophoresis separation. By marking the HCP protein spots on the two-dimensional electrophoresis map that react with the antibody, the specificity and coverage of the antibody can be determined. Combined with mass spectrometry, the specific types of HCP covered by the antibody and important HCP that may be missed can be accurately identified. The 2D-DIGE technique offers higher resolution, reproducibility, and accuracy compared to traditional two-dimensional electrophoresis, and has been widely used to compare HCP expression levels under different production batches or conditions to evaluate process consistency and control.
Biotech Park 2D-DIGE Antibody Coverage Analysis
Biotech Park (BTP) is certified by both CNAS and ISO9001 quality systems; the company has established a 2D-DIGE technology platform to ensure that all HCP antibody coverage analyses meet your needs. Through Biotech Park's 2D-DIGE antibody coverage analysis service, you can obtain detailed information on HCP types and expression levels, effectively screen efficient HCP antibodies suitable for your production process, significantly improve the controllability and quality of biopharmaceutical production, reduce patient drug use risks, and shorten the time from R&D to market. BTP also offers mass spectrometry options, allowing you to gain a deeper understanding of different types of HCP that may occur during production. Our multidisciplinary team can customize project plans free of charge to meet various experimental needs, and we look forward to collaborating with you.
Bilingual Project Report
In the technical report, Biotech Park will provide you with a detailed bilingual (Chinese and English) technical report, which includes:
1. Experimental Procedures (Chinese and English)
2. Relevant Experimental Parameters (Chinese and English)
3. Detailed Information on 2D-DIGE Antibody Coverage Analysis
4. Electrophoresis Images
5. Raw Data
2D-DIGE Antibody Coverage AnalysisOne-stop Service
You only need to place an order and send samples
Biotech Park One-stop Service Completion: Sample Processing - Machine Analysis - Data Analysis - Project Report
Related Services
Host Cell Protein (HCP) Analysis Service
2D-DIGE Quantitative Proteomics
Two-dimensional Gel Electrophoresis Service
Product-related Impurity Analysis
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