Two-Dimensional Gel Electrophoresis Services

Two-dimensional gel electrophoresis (2-DE), also known as two-dimensional electrophoresis, is a powerful and widely used method. Two-dimensional gel electrophoresis combines isoelectric focusing and SDS-PAGE methods. Proteins are first separated by their isoelectric points (pI) through isoelectric focusing, and then separated again by molecular weight using SDS-PAGE. Each protein spot on the two-dimensional gel can be eluted and identified using high-throughput mass spectrometry. Therefore, two-dimensional gel electrophoresis is particularly suitable for comparing protein profiles between different tissues, conditions, or between control and experimental samples.

Introduction to Biotechpac's Two-Dimensional Gel Electrophoresis Service

Biotechpac Biotechnology offers two-dimensional gel electrophoresis services. This service can separate complex mixtures containing thousands of different proteins for subsequent quantitative analysis.

Flowchart of Two-Dimensional Gel Electrophoresis Service

1.Sample Preparation: The preparation process depends on the sample type.
2.First Separation by Isoelectric Focusing: Load the protein mixture onto a pH gradient gel, where proteins migrate under the influence of an electric field.
3.BySDS-PAGEConduct Two-Dimensional Separation: This method typically includes three steps: preparing the gel, equilibrating the pH gradient strips in SDS buffer, and electrophoresis.
4.Staining: Use various staining methods, such as silver staining and Coomassie Brilliant Blue staining, to stain the proteins.
5.Result Analysis: In addition to protein separation, it also allows for the determination ofprotein molecular weight, isoelectric point, and content, among other information.

Sample Requirements

We accept various types of samples, including but not limited to:
• Purified proteins
• Frozen cell pellets or tissues
• Cells (e.g., blood cells) or tissues
• Serum/plasma/urine
• Cell cultures
• Fermentation cultures
• Microorganisms
• Plants
• Others

Advantages of Two-Dimensional Gel Electrophoresis Services

High throughput: Thousands of proteins can be analyzed in a single two-dimensional gel electrophoresis.
High resolution: Proteins are resolved by isoelectric points and molecular weight, with the ability to characterize proteins through post-translational modifications affecting their charge states.
Provides various computer-based tools: We have tools such as SameSpots, Delta2D, ImageMaster for detecting and quantifying protein spots.
Lower cost: Two-dimensional electrophoresis is relatively less expensive compared to mass spectrometry-based methods.
Flexibility: Biotechpac Biotechnology offers customizable analysis services, designing the optimal experimental plan to achieve your research goals within budget.

Applications of Two-Dimensional Gel Electrophoresis

• Protein classification
• Protein isoform distribution analysis
• Protein quantification
• Post-translational modifications of proteins
• Quality control of proteins and antibodies
• Host cell protein analysis
• Downstream analysis, such as protein immunoblotting
• Developmental genetics, disease etiology, biomarker and target discovery

With years of experience, Biotechpac Biotechnology offers high-resolution, high-throughput two-dimensional gel electrophoresis services for protein analysis. The two-dimensional gel electrophoresis analysis service allows direct observation of protein changes and post-translational modifications, as well as the discovery and detection of biomarkers and new drugs. We also offer customizable services based on your project requirements.