With the popularization of flow cytometry and solid-phase technology and the revolutionary development of bead-based multiplex detection technology, flow cytometry can also be used for qualitative and quantitative analysis of soluble proteins. Unlike traditional ELISA technology, which can only detect one indicator, flow-based multiplex detection technology allows for the simultaneous detection of multiple indicators from a single specimen, greatly improving the efficiency of researchers and enabling multi-index analysis of rare and precious samples. Representative technologies include Luminex, BD CBA (cytometric bead array system, CBA), and eBioscience's FlowCytomix technology. Baite Parker uses BD's CBA technology and FlowCytomix technology to provide researchers with multi-parameter cytokine or other soluble molecule detection services.
Detection Principle:
The detection principle of this technology is the same as the sandwich immunoassay. Fluorescent beads are coated with antigen-specific antibodies. The bead mixture coated with antibodies is incubated with the sample. The analyte (such as cytokines) in the sample binds to the antibodies on the beads. A biotin-labeled antibody mixture specifically binds to the analyte captured by the antibodies (beads). Finally, Streptavidin-PE is added to bind with biotin for fluorescence detection. The flow cytometer differentiates bead populations based on bead size and fluorescence intensity. Two sizes of beads (4.4μm, 5.5μm) are internally labeled with 9 and 11 different intensities of fluorescent dyes (far-red fluorescence, which can be excited by argon, helium-neon, or even ultraviolet light sources, emission wavelength 690nm), forming 20 different types of beads that can be simultaneously identified and distinguished by the flow cytometer, thus allowing up to 20 indicators to be detected from a single specimen.
Flow Cytokine Detection - Baite Parker
Baite Parker provides the following flow-based multiplex detection services:
Compared with traditional ELISA detection, using flow cytometry for multiplex detection has the following advantages:
Advantages of Flow Cytokine Detection
Antibody Selection
Currently, there are hundreds of antibodies suitable for this technology, which can be randomly combined and developed according to different research needs. We have developed a series of flow kits from common factor combinations, significantly reducing the time required for antibody customization. For human samples, rat samples, and mouse samples, single-factor and multi-factor flow kits are available as shown in the table below (more antibodies are under development, please call for consultation):
Mouse Sample Single-Factor Flow Kit
Rat Sample Multi-Factor Flow Kit
Rat Sample Single-Factor Flow Kit
About Samples
1. Only 25µL of sample is needed, while conventional ELISA requires 50µL or 100µL of sample.
2. Suitable for serum, plasma, cell culture supernatant, and various body fluids.
3. Body fluid samples should be stored in a -80°C freezer and transported with dry ice.
Bilingual Project Report:
In the final technical report, Baite Parker will provide you with a detailed bilingual technical report in Chinese and English, including:
1. Experimental Procedures (Chinese and English)
2. Raw Data (Fluorescence Emission Spectrum)
3. Technical Report
4. Absolute Content of Each Detection Indicator (Excel Spreadsheet)
Flow-Based Multiplex Detection One-Stop Service
You only need to place an order and send samples
Baite Parker One-Stop Service completes: Sample Processing - On-Machine Analysis - Data Analysis - Project Report
