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SEC-MALS Molecular Weight Detection

SEC-MALS (Size Exclusion Chromatography - Multi-Angle Light Scattering) is a combined techniqueSize exclusionchromatography (SEC) and multi-anglelaserscattering (MALS) for accurate measurement of molecular mass and molecular sizeabsolute determinationtechnique.SECis based onthe different times proteins of varying molecular weights take to pass through a porous medium, whichdiffer in timeto achieve sample separation, while MALS technology calculates the molecular mass and size by measuring the scattering intensity of each molecule at different angles. SEC-MALSanalysis canprovide accurate information about molecular mass, size, shape, and assembly state,is animportant tool for an in-depth understanding of the structure and function of biological macromolecules..

Basic Principles of SEC-MALS Molecular Weight Detection

SEC-MALScan directly measure the molecular weight and size of macromolecules and nanoparticles in solution without relying on column calibration and standards, widely applied inthe structural and dynamic study of proteins, glycans, polymers, nanoparticles,and in the study of aggregation and denaturation of biopharmaceuticalswidely applied.BTP (Biotechnology Park) has established a molecular weight determination platform based on high-performance liquid chromatography and has been certified by CNAS/ISO9001 dual quality system. We can provide you with direct SEC-MALS molecular weight detection services, including method development, validation, and transfer services.

ExperimentInstrument

High-performance liquid chromatography (UV detector): Waters 2695/2996

Technical Application

Can detect the molecular weight of mixed samples, suitable for more complex mixed samples.

Case Illustration

Introduce the prepared sample into the SEC system. Large molecules are eluted first as they cannot enter the pores, while small molecules are eluted later as they enter the interior of the pores. The eluted sample from the SEC column then flows into the MALS detector. The MALS detector calculates the absolute molecular weight of the sample by scattering laser light across the sample and measuring the intensity of the scattered light from multiple angles. Finally, the MALS raw data is analyzed by data analysis software to obtain molecular weight and aggregation information of the sample.

Sample chromatographic detection schematic diagram, with the horizontal axis representing retention time and the vertical axis representing UV signal intensity

Molecular weight detection result schematic diagram, horizontal axis represents time, vertical axis represents molar mass

Sample Molecular Weight Distribution Table

Bilingual Project Report

In the technical report, BTP provides a detailed bilingual (Chinese and English) technical report, which includes:

1. Experimental steps (Chinese and English)
2. Relevantchromatographyparameters (Chinese and English)
3. Detailed information on SEC-MALS molecular weight detection
4. Chromatographyimages
5. Raw data

One-stop SEC-MALS Molecular Weight DetectionService

You only need to place an order and send the sample
BTP one-stop service completes: sample processing - on-machine analysis - data analysis - project report