Peptide Map Analysis
With the advancement of modern pharmaceutical industry, peptide mapping analysis has become a key step in the characterization of biological products and an important means of quality control. Peptide mapping uses specific chemical reagents or enzymes to specifically degrade proteins into peptide fragments, which are then separated and identified by reliable methods, and compared with a reference treated by the same method for result determination. Depending on the instruments used, peptide mapping analysis can be divided into HPLC peptide mapping analysis based on high-performance liquid chromatography and HPLC mass peptide mapping analysis based on mass spectrometry.
Beijing BiotechPack Co., Ltd. (BTP) has established HPLC peptide mapping and HPLC mass peptide mapping analysis platforms based on high-performance liquid chromatographs and liquid chromatography-mass spectrometry.,BiotechPackhas been certified by CNAS/ISO9001 dual quality system;provides youHPLC peptide mapping and HPLC mass peptide mapping analysis services, including comprehensive services for method development, validation, and transfer.
BiotechPack Peptide Mapping Method Validation Content
Experimental Instruments
• Liquid Chromatograph: UltiMate3000
• High-performance liquid chromatography (UV detector) (2695/2996)
• High-resolution mass spectrometer: AB SCIEX 5600+
Technical Applications
• Evaluate batch consistency of production processes and stability of cell matrix expression used in production in identity tests for product release inspection;
• Detect and monitor changes in single amino acids, such as oxidation, deamidation, and other degradations;
• Directly detect common biological product modifications and variations such as N-terminal cyclization, C-terminal lysine processing, N-glycosylation, etc.
Common Questions
Question 1: What samples are suitable for peptide mapping analysis?
Answer:Samples suitable for peptide mapping analysis usually have a known theoretical reference amino acid sequence or a reference sample available. For the former, we generally use mass spectrometry for HPLC mass peptide mapping analysis; for the latter, we use reversed-phase high-performance liquid chromatography for HPLC peptide mapping analysis.
Bilingual Project Report
In the technical report, BiotechPack provides you with a detailed bilingual technical report in Chinese and English, which includes:
1. Experimental steps (in Chinese and English)
2. Relevant mass spectrometry parameters (in Chinese and English)
3. Peptide MappingDetailed Information
4. Mass spectrometry images
5. Raw data
One-stop Peptide Mapping Service
You only need to place an order and send the sample
BiotechPack's one-stop service completes: sample processing - machine analysis - data analysis - project report
Related Services
HPLC Mass Peptide Mapping Analysis
Peptide Mass Fingerprint Mapping Analysis Service Peptide Segment Coverage/Peptide Mapping Analysis
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