HPLC Quality Peptide Map Analysis
HPLC peptide mapping analysis combines the separation capability of high-performance liquid chromatography (HPLC) with the qualitative and quantitative capabilities of mass spectrometry (MS), utilizing an HPLC tandem high-resolution mass spectrometry (LC-MS/MS) platform for detection.Iteffectively separates peptide fragments using HPLC technology, and then uses MS to measure mass and charge, identifying the peptide fragments corresponding to each chromatographic peak and assigning them in the peptide map, forming a characteristic fingerprint peptide map.HPLC peptide mapping analysis can provide comprehensive information about the sample under investigation.
BTP Bio-Technology has established an HPLC peptide mapping analysis platform based on the principles of peptide mapping analysis, combining high-performance liquid chromatography and high-resolution mass spectrometers.; BTP Bio-Technologyhas been certified with dual quality systems CNAS/ISO9001, providing you with a one-stopHPLCqualitypeptide mappingservice,welcome to consult for free to learn more details!
Experimental Instruments
• Liquid Chromatograph: UltiMate3000
• High-resolution Mass Spectrometer: AB SCIEX 5600+
Technical Applications
• Use mirror images to determine stability between batches or whether there are differences between each batch and the standard, and accurately identify inconsistent positions;
• Can be further used for qualitative analysis of protein variants, disulfide bond positioning, glycosylation site analysis, protein modification site determination, etc.
Case Illustration
After proteolytic digestion, the sample is separated by high-performance liquid chromatography, and the mass spectrometer collects data to obtain peptide map results, as shown in the figure:
Total Ion Chromatogram of the Sample
The collected mass spectrometry raw data is analyzed by software, and the identified peptide information and the relationship with the chromatographic peaks are shown in the following table:
Table of Identified Peptide Information and Corresponding Chromatographic Peaks for the Sample
Sequence Coverage Map
Bilingual Project Report
In the technical report, BTP will provide you with a detailed bilingual (Chinese and English) technical report, which includes:
1. Experimental Steps (Chinese and English)
2. Related Mass Spectrometry Parameters (Chinese and English)
3. Detailed Information on HPLC Peptide Mapping Analysis4. Mass Spectrometry Images
5. Raw Data
HPLC Peptide Mapping Analysis
One-Stop ServiceYou only need to place an order and send the samples
BTP's one-stop service completes: sample processing - machine analysis - data analysis - project report
Related Services
Peptide Mapping
Protein Peptide Mapping Determination
Peptide Coverage/Peptide Map Analysis
How to order?
