HPLC Peptide Map Analysis
HPLC Peptide Mapping Analysisis a routine identification test,primarily used for analyzing and identifying peptide fragments generated from protein enzymatic digestion.The basic principle is to enzymatically digest protein samples into multiple peptide segments, which are then separated byreversed-phasehigh-performance liquid chromatography (RP-HPLC).After separation through the chromatography column, peptides are detected using a UV detector at a wavelength range of 200-230nm (typically 214nm), and a chromatogram is recorded.Finally,the chromatogram of the test sample is compared to that of a reference standard, achievingaccurate identification and quantificationof the target peptide segments,ultimately used in product release tests for identification, assessing inter-batch consistency of the production process, and stability of cell matrix expression used in production.
Reversed-phase high-performance liquid chromatography (RP-HPLC) is a high-resolution, sensitive analytical method that provides rich information on proteins and peptides and is the standard method for protein and peptide analysis and characterization.BTP Biotech has established a peptide mapping analysis platform based on Waters' HPLC, equipped with comprehensive method development, validation, and testing capabilities.BTP Biotechis dual-certified with CNAS/ISO9001 quality systems;providing you with one-stopHPLC peptide mappingservices,welcome to consult for free to learn more details!
Laboratory Instruments
• High-performance liquid chromatography (UV detector) (2695/2996)Case Illustration
Samples after proteolytic digestion are separated by high-performance liquid chromatography, and the UV detector collects data to obtain peptide mapping results, as shown in the chromatogram below:
Figure 1 Chromatogram of Reference Standard
Figure 2 Chromatogram of Sample
Based on the analysis of the above chromatograms, it can be observed that the peak shape and retention time of the sample peptide map are basically consistent with those of the reference sample.
Bilingual Project Report
In the technical report, BTP Biotech provides you with a detailed bilingual report in Chinese and English, which includes:
1. Experimental Steps (in Chinese and English)
2. Relevant Mass Spectrometry Parameters (in Chinese and English)
3. HPLC Peptide MappingDetailed Information
4. Mass Spectrometry Images
5. Raw Data
HPLC Peptide MappingOne-stop Service
All you need to do is place an order and send the sample
BTP Biotech provides one-stop service: sample processing - machine analysis - data analysis - project report
Related Services
HPLC Quality Peptide Mapping Analysis
Protein Peptide Mapping Determination
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