Oxylipidomics
Oxidized lipids are a class of bioactive molecules formed by the oxidation of unsaturated fatty acids (such as linolenic acid, arachidonic acid, and eicosatetraenoic acid), including prostaglandins, leukotrienes, lysine ketones, various enols and ketones. Oxylipidomics is an emerging field that studies the composition, distribution, transformation, and function of oxidized lipids in organisms. As crucial factors in cell signaling and disease pathogenesis, changes in oxidized lipids in organisms are essential for understanding the mechanisms of various diseases, such as inflammation, cardiovascular diseases, immune responses, cell growth regulation, cancer, and neurodegenerative diseases. Therefore, accurate analysis and assessment of the composition and changes of oxidized lipids are of significant value for disease prevention, diagnosis, and treatment.
The core of oxylipidomics is the identification and quantification of oxidized lipids. Due to the chemical diversity and dynamic nature of oxidized lipids, highly sensitive and precise mass spectrometry methods, especially liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS), are required to achieve detailed qualitative and quantitative analysis of the types, structures, and concentrations of oxidized lipids.
Lipid oxidation process
BTP (Bio-Technology Park) offers seven analytical platforms and is dual-certified by CNAS/ISO9001 quality systems. The company provides oxylipidomics analysis services ranging from basic qualitative analysis to in-depth quantitative analysis based on high-resolution LC-MS and GC-MS platforms. BTP has a professional and experienced team that can provide you with free, efficient, and personalized solutions to meet your various needs in disease research, new drug development, and nutrition. Feel free to contact us at any time for more information and services regarding oxylipidomics analysis!
OxylipidomicsAnalysisProcess
1. Lipid extraction: Use organic solvents (such as methanol, chloroform, etc.) to extract lipids from samples.
2. Chromatographic separation: Choose liquid chromatography (LC) or gas chromatography (GC) based on the physical properties of the lipid extracts for separation.
3. Mass spectrometry analysis: Introduce the chromatographically separated lipids into a mass spectrometer for ionization (such as electrospray ionization ESI), time-of-flight measurement, and mass analysis (such as time-of-flight mass spectrometry TOF, quadrupole mass spectrometry, etc.) detection.
4. Data processing and analysis: Use software to process and analyze mass spectrometry data, compare mass spectrometry data with known databases, and identify and quantify lipids.
Mass spectrometry analysis process of oxylipidomics
OxylipidomicsAnalysisTechnical Advantages
1. High sensitivity and accuracy
The application of mass spectrometry technology enables oxylipidomics analysis to detect extremely small quantities of lipid molecules and accurately determine their mass and structure.
2. Comprehensiveness and dynamism
Comprehensively analyze all lipids in the sample and analyze the dynamics of lipid changes.
3. Wide applicability
Applicable to various biological samples such as blood, urine, cells, and tissues, making oxylipidomics applicable to multiple research fields (such as basic biology, medicine, food science, and environmental science, etc.).
4. Quantitative analysis of lipids
Using internal and external standards, oxylipidomics can achieve quantitative analysis of lipids.
About Samples
|
Sample Types |
Blood |
Urine |
Saliva |
Tissue |
Cells |
Culture medium |
|
Sample Quantity |
1-10ml |
10-1000ml |
10-1000ml |
1-104mg |
106-108units |
10-100ml |
MediumEnglish Project Report
In the technical report, BTP will provide you with a detailed bilingual technical report in both Chinese and English, including:
1. Experimental steps (in Chinese and English)
2. Relevant mass spectrometry parameters (in Chinese and English)
3. Detailed information on oxylipidomics analysis
4. Mass spectrometry images
5. Raw data
OxylipidomicsAnalysisOne-stop Service
You just need to place an order and send the samples
BTP's one-stop service completes: sample processing, on-machine analysis, data analysis, and project report
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