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2D-DIGE Quantitative Proteomics

2D-DIGE (Two-dimensional fluorescence difference gel electrophoresis) is a new quantitative proteomics technique developed from traditional two-dimensional electrophoresis. The principle of separating mixed proteins in 2D-DIGE is consistent with two-dimensional electrophoresis, utilizing differences in the isoelectric point and molecular weight to separate protein mixtures. At the same time, applying the sensitivity of fluorescent dyes and internal standards, it significantly outperforms traditional two-dimensional electrophoresis in quantitative proteomics research. The fluorescent dyes used in DIGE are Cy2, Cy3, and Cy5, which can react with the amino group of the lysine side chain of proteins to label them. The isoelectric point and molecular weight of labeled proteins remain unaffected. After mixing labeled proteins in equal amounts for two-dimensional electrophoresis, differences between gels can be eliminated through Cy2 internal standard matching, while changes in protein expression levels are reflected by the intensity of different fluorescence from Cy3 and Cy5. As a classic method in quantitative proteomics, 2D-DIGE is widely applicable and suitable for various samples.

Blundon, M. Methods Mol Biol. 2019.

Offered by Biotech PackSDS-PAGEand 2D-DIGE electrophoresis services combined with Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform and nanoLC-MS/MS nano chromatography to provide one-stop qualitative and quantitative proteomics services for researchers.

About the samples

Both liquid and solid samples are acceptable

Application range

Suitable for various samples

Chinese/English Project Report

In the technical report, Biotech Pack will provide you with a detailed bilingual technical report in Chinese and English, which includes:

1. Experimental steps (Chinese and English)
2. Related experimental parameters (Chinese and English)
3. Gel images and mass spectrometry images
4. Raw data
5. 2D-DIGE quantitative proteomics analysis results

2D-DIGE Quantitative Proteomics One-Stop Service

You only need to place an order and send the samples

Biotech Pack one-stop service includes: sample processing, on-machine analysis, data analysis, and project report completion