SWATH (Sequential Window Acquisition of all Theoretical fragment ions) is a novel mass spectrometry acquisition mode technology jointly developed by Dr. Ruedi Aebersold's team at ETH Zürich and AB SCIEX. As a DIA (Data Independent Acquisition) technique, SWATH divides the entire mass spectrometry scanning mass range into several small windows and sequentially fragments all ions in each window. This enables high-speed primary MS scanning of all peptide ions in the scanning range followed by secondary MS/MS analysis. Consequently, SWATH can quantitatively measure almost all detectable molecules in complex samples. SWATH combines the high-throughput detection features of shotgun proteomics with the advantages of precise quantitative analysis, representing a truly comprehensive mass spectrometry detection approach, MS/MS ALL. BGI Park employs the AB SCIEX Triple-TOF 5600 plus high-resolution mass spectrometry system, which features fast scanning speed and the quantitative sensitivity capabilities of a tandem quadrupole mass spectrometry system. It integrates high resolution, accurate mass stability, high sensitivity, and high-speed scanning characteristics of the mass spectrometry system. We offer a SWATH quantitative proteomics analysis service package, where you only need to send us your samples, and we will handle all subsequent matters, including protein extraction, proteolytic digestion, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.
SWATH Quantitative Proteomics Service Technology Platform
SWATH Quantitative Proteomics Service Technology Platform
Features of SWATH Technology
• Quantitative identification of all molecules in complex samples, including low-abundance proteins and peptides
• High quantitative accuracy: comparable to MRM technology
• High throughput: can quantitatively detect over 2000 proteins in a single experiment
• Complete and comprehensive information storage, capable of storing data for every peak in MS and MS/MS, eliminating the need for repeated detection of the same sample
• No need to pre-tune the sample measurement methods as required in MRM technology
Advantages of SWATH/DIA
A major issue in quantitative analysis of complex samples often arises from the interference of high-abundance proteins, making it difficult to identify low-abundance proteins. Many DDA (Data Dependent Acquisition) mass spectrometry identification methods prioritize selecting peptides with strong MS signals for secondary fragmentation, making it challenging for low-abundance peptides to be chosen for secondary analysis. In contrast, SWATH/DIA analyzes all molecules in the sample indiscriminately and stores all the MS, MS/MS data of all peptides in one go, ensuring no potentially important data is missed. Overall, SWATH significantly enhances the reliability of quantitative analysis and efficiently determines proteins with very low abundance in complex samples.
Comparison of DDA and SWATH/DIA Mass Spectrometry Analysis
Additionally, compared toMRMwhich requires optimizing the detection method based on the target molecules to be detected, SWATH provides unbiased analysis of all molecules without the need to optimize detection methods in advance. Optimization can be done based on the already acquired detection data. Therefore, SWATH analysis can significantly shorten the cycle of detection and analysis for you.
SWATH Quantitative Proteomics Service Sample Requirements
SWATH Quantitative Proteomics Service Sample Requirements
Bioinformatics Analysis
• SWATH spectrum analysis, protein data quality assessment
• PCA analysis of multi-component samples
• Functional annotation of identified proteins: GO function annotation, KEGG function annotation, and COG function annotation
• Statistical analysis of differentially expressed proteins: Venn diagram, Volcano Plot
• Clustering analysis of differentially expressed proteins: hierarchical clustering, K-means clustering
• Interaction analysis of differential proteins: protein interaction network analysis based on the STRING database
Bilingual Project Report
In the final technical report, BGI Park Biotechnology will provide you with a detailed bilingual technical report in both Chinese and English, which includes:
1. Experimental procedures (in Chinese and English)
2. Relevant mass spectrometry parameters (in Chinese and English)
3. Mass spectrometry images
4. Raw data
5. Protein differential level analysis
6. Bioinformatics analysis
SWATH Quantitative Proteomics One-Stop Service
You only need to place an order and send the samples
BGI Park's one-stop service completes: sample processing - on-machine analysis - data analysis - project report
