MRM/PRM Quantitative Proteomics Analysis

MRM (Multiple Reaction Monitoring) is a targeted quantitative proteomics research method focusing on specific protein molecules. MRM selects data for mass spectrometry acquisition based on target molecule information, capturing signals that meet the target ion criteria and eliminating interference from non-compliant ion signals. MRM mass spectrometry analysis involves three stages: 1. Selecting precursor ions that specifically match the target molecule through MS; 2. Fragmenting these precursor ions to remove interference from other ions; 3. Collecting mass spectrometry signals only for selected specific MS/MS2 ions. MRM mass spectrometry technology is a highly precise technique for protein quantification, making it an excellent method for accurate quantitative analysis of multiple target proteins in complex samples. By using isotopically labeled target peptides as internal references, absolute protein quantification can be achieved.

BaiTaiPike Company utilizes the AB SCIEX TripleTOF 5600, AB SCIEX Triple Quad™ 5500, Q Exactive, and Fusion mass spectrometry platforms in combination with Nano-LC to offer MRM/PRMtargeted quantitative proteomics analysis service package. You only need to provide the information of the target proteins to be studied, and we offer a one-stop MRM quantitative protein analysis service. This includes MRM method development and optimization, specific peptide selection, isotopically labeled peptide synthesis, mass spectrometry analysis, raw data analysis, and bioinformatics analysis.

BTP-MRM/PRM quantitative proteomics analysis platform

AB SCIEX TripleTOF 5600, AB SCIEX Triple Quad™ 5500, Q Exactive, Fusion

MRM/PRM quantitative proteomics analysis experimental process

MRM-PRM quantitative proteomics analysis experimental process

MRM-PRM quantitative proteomics analysis experimental process 2

Characteristics of MRM technology

• High sensitivity, selecting ions consistent with target ions through two stages of mass spectrometry, excluding interfering ions, significantly improving signal-to-noise ratio and enhancing detection accuracy of target ions
• High throughput, capable of identifying up to 200 proteins in one go
• Capable of absolute quantitative analysis without relying on antibodies
• High precision identification of low-abundance proteins, with a quantitative range spanning 4 orders of magnitude

Application fields of MRM technology

• Validation of results from label-free and other non-targeted proteomics studies
• Absolute quantitative studies on multiple proteins/peptides simultaneously
• Research on protein family changes with high homology but lacking specific recognition antibodies
• Quantitative research on post-translational modifications of proteins
• Absolute quantitative research on biological disease targets

Sample requirements for MRM analysis

Sample requirements for MRM-PRM quantitative proteomics analysis

Bioinformatics analysis for MRM technology services

• MRM chromatogram analysis and protein data quality assessment
• PCA analysis of multi-component samples
• Functional annotation of identified proteins: GO functional annotation, KEGG functional annotation, and COG functional annotation
• Statistical analysis of differentially expressed proteins: Venn diagram, Volcano Plot
• Clustering analysis of differentially expressed proteins: hierarchical clustering, K-means clustering
• Interaction analysis of differential proteins: protein interaction network analysis based on STRING database

Applications of SRM/MRM

• Validation of iTRAQ differential proteins;
• Validation of subsequent products of label-free differential proteins;
• Absolute quantification of peptides and proteins;
• Quantification of disease markers and establishment of diagnostic models;
• Quantification of phosphorylated proteins and methylated proteins;
• Quantification of other post-translationally modified proteins;
• Pathway quantitative analysis.

Chinese/English project report

In the technical report, BaiTaiPike provides a detailed bilingual Chinese and English version, including:

1. Experimental steps (Chinese and English)
2. Related mass spectrometry parameters (Chinese and English)
3. Mass spectrometry images
4. Raw data
5. Protein differential level analysis
6. Bioinformatics analysis

One-stop service for MRM quantitative proteomics

You only need to place an order and send samples

BaiTaiPike's one-stop service completes: sample processing - on-machine analysis - data analysis - project report

Related services

Parallel Reaction Monitoring (PRM) service
DIA-PRM proteomics
Absolute Quantitative Analysis (AQUA)
Targeted proteomics
Quantitative proteomics analysis
4D proteomics
Analysis of post-translationally modified proteomes
Label-free quantitative proteomics analysis
Label-based protein quantification technologies - iTRAQ, TMT, SILAC
TMT/iTRAQ/MultiNotch quantitative proteomics analysis
DIA quantitative proteomics
SWATH quantitative proteomics service
2D-DIGE quantitative proteomics
Bioinformatics analysis of proteomics