DIA-PRM Proteomics

DIA (data-independent acquisition) refers to a data-independent scanning mode, which is a holographic mass spectrometry data acquisition mode based on the Orbitrap. Compared to DDA (data-dependent acquisition), DIA technology divides the entire mass spectrometry scan range into several windows, selects, fragments, and detects all ions in each window, offering better accuracy and reproducibility. PRM (parallel reaction monitoring) is an ion monitoring technique based on high-resolution and high-precision mass spectrometry. It is currently the mainstream method for targeted proteomics data acquisition, achieving targeted relative or absolute quantification of target proteins/modification peptides by selectively detecting specific peptides or target peptides (such as post-translational modification peptides). Combining DIA and PRM for proteomics research enables detection of large sample sizes or complex samples and precise quantification of target proteins, especially improving the detection rate of low-abundance target proteins.

Biotech Pack uses Thermo's latest Obitrap Fusion Lumos mass spectrometer combined with Nano-LC nanoliter chromatography technology to provide DIA+PRM proteomics analysis service packages. You only need to send your samples to us, and we will handle all subsequent matters, including protein extraction, protease digestion, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.

DIA+PRM Proteomics Analysis Workflow

DIA+PRM Proteomics

Application Areas

It can be applied in various biological science research fields such as agriculture, forestry, environment, food, and pharmaceuticals to construct biological sample information databases and discover biomarkers.

Chinese/English Project Report

In the final technical report, Biotech Pack will provide you with a detailed bilingual (Chinese and English) technical report, which includes:

1. Experimental Procedures (Chinese and English)
2. Related Mass Spectrometry Parameters (Chinese and English)
3. Mass Spectrometry Images
4. Raw Data
5. Protein Differential Level Analysis
6. Bioinformatics Analysis

DIA+PRM Proteomics One-Stop Service

You only need to place an order and send your samples

Biotech Pack One-Stop Service Completion: Sample Processing - On-Machine Analysis - Data Analysis - Project Report

Related Services

DIA Quantitative Proteomics
Parallel Reaction Monitoring (PRM) Services
Proteomics Analysis Strategy
PCT-DIA Proteomics
MRM/PRM Quantitative Proteomics Analysis
SWATH Quantitative Proteomics Services
Quantitative Proteomics Analysis
Targeted Proteomics
Post-Translational Modification Proteomics Analysis
4D Proteomics
Proteomics Bioinformatics Analysis