PCT-DIA Proteomics

PCT (Pressure Cycling Technology) is an efficient biological sample preparation technology developed by the American company PBI. It uses alternating cycles between atmospheric and ultra-high (liquid) pressure (35,000 PSI or higher) to repeatedly break cells, enabling the extraction of important biomolecules like proteins from various samples, including human, animal, plant, and microbial cells and tissues. DIA (data-independent acquisition) is a holistic mass spectrometry data acquisition mode based on the electrostatic field Orbitrap. Compared to DDA (data-dependent acquisition), DIA technology divides the entire mass spectrometry scan range into several windows, selecting, fragmenting, and detecting all ions within each window, offering better accuracy and reproducibility. The combination of PCT and DIA technologies enables proteomic research on paraffin-embedded or formalin-fixed tissue samples, as well as trace samples.

BaiTai-Peike Biotechnology uses Thermo's latest Obitrap Fusion Lumos mass spectrometer combined with Nano-LC nanoscale chromatography technology to offer the PCT+PRM proteomics analysis service package. You only need to send us your samples, and we will handle all subsequent project matters, including protein extraction, protein digestion, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.

PCT+DIA Proteomics Analysis Workflow

Guo, T. et al. iScience. 2019.

PCT+DIA Proteomics

Bilingual Project Report (Chinese/English)

In the final technical report, BaiTai-Peike will provide you with a detailed bilingual (Chinese and English) technical report, including:

1. Experimental Procedures (Chinese/English)
2. Relevant Experimental Parameters (Chinese/English)
3. Mass Spectrometry Images
4. Raw Data
5. Protein Differential Level Analysis
6. Bioinformatics Analysis

PCT+DIA Proteomics One-stop Service

You only need to place an order and send the samples
BaiTai-Peike One-stop Service Completion: Sample Processing - On-machine Analysis - Data Analysis - Project Report

Related Services

DIA Quantitative Proteomics
Proteomics Analysis Strategy
DIA-PRM Proteomics
SWATH Quantitative Proteomics Service
Quantitative Proteome Analysis
Targeted Proteomics
Post-translational Modification Proteome Analysis
Proteomics Bioinformatics Analysis