DIA Quantitative Proteomics

DIA (data-independent acquisition) refers to a data-independent scanning mode, which is a holographic mass spectrometry data acquisition mode based on the Orbitrap electrostatic field. After the first-level mass spectrometry detection, DIA fragments all parent ions within a specific mass-to-charge ratio range, collects fragment ions of all parent ions, and quickly scans all fragment ions within adjacent wide parent ion windows, thereby performing qualitative and quantitative analysis of proteins. Compared to DDA (data-dependent acquisition mode), DIA offers better accuracy and reproducibility. SWATH technology is a type of DIA technology that combines the high-throughput detection characteristics of shotgun proteomics with the advantages of PRM precise quantitative analysis, enabling quantitative measurement of almost all detectable molecules in complex samples.

BaiTai Piker Biotechnology's DIA quantitative proteomics service uses the AB SCIEX Triple-TOF 5600 plus high-resolution mass spectrometry system, featuring fast scanning speed and the quantitative sensitivity capabilities of a quadrupole mass spectrometry system. It integrates high resolution, accurate mass stability, high sensitivity, and high-speed scanning of the mass spectrometry system. You only need to send your samples to us, and we will handle all subsequent project matters, including protein extraction, proteolysis, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.

DIA Quantitative Proteomics Analysis Process

DIA Quantitative Proteomics

Application Fields

It can be applied in various biological sciences such as agriculture, forestry, environment, food, and medicine for constructing biological sample information libraries and discovering biomarkers.

Bilingual Project Report in Chinese/English

In the final technical report, BaiTai Piker will provide you with a detailed bilingual technical report in Chinese and English, which includes:

1. Experimental Steps (Chinese/English)
2. Relevant Mass Spectrometry Parameters (Chinese/English)
3. Mass Spectrometry Images
4. Raw Data
5. Protein Differential Level Analysis
6. Bioinformatics Analysis

One-Stop DIA Quantitative Proteomics Service

You only need to place an order and send the samples

BaiTai Piker's one-stop service completes: Sample processing - On-machine analysis - Data analysis - Project report

Related Services

Quantitative Proteomics Analysis
4D Proteomics
SWATH Quantitative Proteomics Service
DIA-PRM Proteomics
PCT-DIA Proteomics
Label-Free Quantitative Proteomics Analysis
Label-Based Protein Quantification Techniques - iTRAQ, TMT, SILAC
TMT/iTRAQ/MultiNotch Quantitative Proteomics Analysis
TMT-Based Proteomics Analysis
2D-DIGE Quantitative Proteomics
Targeted Proteomics
MRM/PRM Quantitative Proteomics Analysis
Absolute Quantification Analysis (AQUA)
Post-Translational Modification Proteomics Analysis
Proteomics Bioinformatics Analysis