AAE-nanoLC-MS/MS Antibody Coverage Analysis
In the development and production of antibody drugs, host cell protein (HCP) antibody coverage analysis is a key technology crucial for ensuring drug quality and biological activity. Affinity Antibody Extraction (AAE) combined with nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) provides a powerful tool for HCP antibody coverage analysis. AAE-nanoLC-MS/MS enriches HCPs that can be recognized by antibodies and then compares the enriched HCPs with the original HCPs using the nanoLC-MS/MS platform. Mass spectrometry detection provides both qualitative identification information and relative quantitative information simultaneously. The qualitative identification sensitivity is extremely high, covering 5,000-8,000 proteins, and clearly identifying which proteins are detected and any proteins that require special attention. Relative quantitative information provides clear insight into the enrichment efficiency of each protein, effectively evaluating the accuracy of subsequent ELISA test results. This method is an effective supplement to traditional HCP antibody coverage analysis based on two-dimensional electrophoresis.
BTP-based AAE-nanoLC-MS/MS HCP antibody coverage analysis
Using AAE-nanoLC-MS/MS technology, a comprehensive identification of HCP species covered by antibodies can be achieved, allowing an accurate assessment of the antibody coverage rate. High coverage antibodies help ensure the detection of more potential HCP impurities, which is crucial for the safety evaluation of biopharmaceuticals. Bio-Tek Corporation, certified by CNAS/ISO9001 dual quality systems, utilizes Thermo's latest Obitrap Fusion Lumos mass spectrometer combined with Nano-LC nano-chromatography technology to establish the AAE-nanoLC-MS/MS antibody coverage analysis platform. This platform not only accurately identifies HCP proteins in samples and calculates antibody coverage but also provides relative quantitative information, clearly indicating the enrichment efficiency of each protein, offering you efficient, accurate, and comprehensive customized solutions. We welcome you to consult for free to learn more about AAE-nanoLC-MS/MS antibody coverage analysis services.
Chinese/English Project Report
In the technical report, Bio-Tek will provide you with a detailed bilingual (Chinese and English) version, including:
1. Experimental procedures (in Chinese and English)
2. Relevant mass spectrometry parameters (in Chinese and English)
3. Detailed information on AAE-nanoLC-MS/MS antibody coverage analysis
4. Mass spectrometry images
5. Raw data
AAE-nanoLC-MS/MS antibody coverage analysisOne-stop service
You just need to place an order and send samples
Bio-Tek's one-stop service completes: sample processing, on-machine analysis, data analysis, and project reporting
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