PCR Amplification Based Monoclonal Antibody Sequencing Service

Although hybridoma cells can proliferate indefinitely and express antibodies, long-term cell proliferation and protein expression can lead to mutations in antibody genes, resulting in antibody failure. Therefore, obtaining the monoclonal antibody gene sequence from hybridoma cells is particularly important for large-scale stable expression of monoclonal antibodies. To better meet the needs of research users, Biotech Park has launched a monoclonal antibody gene sequencing service based on PCR amplification technology. Compared to sequencing the amino acid sequence of monoclonal antibodies, gene sequencing can provide more accurate and reliable results and distinguish between amino acids such as leucine/isoleucine, which are difficult to differentiate in mass spectrometry.

Biotech Park offers comprehensive monoclonal antibody gene sequencing services, utilizing professional degenerate primer design and sequencing protocols, optimizing both cost and time to provide customers with fast and accurate sequencing of variable regions and full-length genes. We offer high-quality one-stop services to address all your monoclonal antibody sequencing needs. Customized services are also available according to specific customer requirements.

Experimental Procedure for Monoclonal Antibody Sequencing Service Based on PCR Amplification

Experimental Procedure for Monoclonal Antibody Sequencing Service

Technical Features

• Provides sequence analysis services for antibodies from all common species, capable of sequencing the light chain, heavy chain, or full length
• Efficient and rapid analysis and delivery speed
• Customized primer sets, analysis of multiple DNA clones, ensuring precise and high-quality sequencing analysis
• Sequence analysis of the highly mutable FR1 region of antibodies

About Samples

Sample Form	Cryopreserved monoclonal antibody hybridoma cells
Quantity	1x10^7

Note: Please use an adequate amount of dry ice for sample transportation and choose the fastest possible shipping method to minimize potential degradation during transit.

Bilingual Project Report (Chinese/English)

In the technical report, Biotech Park Biotechnology will provide you with a detailed bilingual report (Chinese/English), including:

1. Experimental Steps (Chinese and English)
2. DNA Sequencing Results
3. Gene Sequence Information of Monoclonal Antibody Light and Heavy Chain Variable Regions
4. DNA Gel Images
5. Raw Data

One-Stop Service for Monoclonal Antibody Sequencing Service Based on PCR Amplification

You only need to place an order and send the samples
Biotech Park's one-stop service completes: Sample Processing - Machine Analysis - Data Analysis - Project Report